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June 12-13, 2019 | Edinburgh, Scotland
8th European Clinical Microbiology and Immunology Congress
&
3rd World congress on Biotechnology
Joint Event
Microbiology: Current Research | Volume: 3 | ISSN: 2591-8036
Colistin resistant Acinetobacter baumanii in a tertiary care hospital of Pakistan: A
retrospective single center study
Syed Bilal Tanvir, Ali Shariq and Hesham S Almoallim
Dar Al Uloom University Hospital, Saudi Arabia
Statement of the Problem : Multidrug resistant (MDR)
A. baumannii is a prominent cause of hospital acquired
infection. It is a gram-negative Coccobacilli mostly
found in soil and is exclusively isolated from hospital
environment, but its natural habitat is still unknown. A.
baumannii is an opportunistic pathogen mostly infecting
immunocompromised patients. It can survive on inanimate
objects for a long period of time, therefore allowing it
to endure in the hospital environment. A surveillance
study conducted in the United States revealed that 10%
of patients admitted in intensive care unit acquired
pneumonia due to A. baumannii . The purposes of this study
were to determine the resistance pattern of A. baumannii
to colistin and carbapenem group of antibiotics in a tertiary
care hospital located in Karachi, Pakistan and compare it
with regional and international resistance pattern.
Methodology & Theoretical Orientation: A total of 705
clinical specimens over the period of July 2016 to June 2017
which included pus, wound swabs, ear swabs, eye swabs,
urine, blood, tracheal aspirates and sputum samples were
collected. All specimens (wound swabs, ear swabs, eye
swabs, sputum, aspirates) were inoculated onto sheep blood
agar, cultures were examined macroscopically for growth.
Antimicrobial susceptibility was performed by Kirbauer disc
diffusion techniques onmuller hilton agar plate and standard
disc zones were measured according to CLSI guideline. SPSS
version 23 was used for statistical analysis. Prevalence and
resistance pattern were deduced as well.
Results : A total of 705 clinical specimens were cultured
and analyzed for antimicrobial resistance pattern. 61%
of the isolates belonged to male patients, while 39% of
the isolates belonged to female patients. The isolates
were tested against amikacin, aztreonam, sulbactam/
cefperazone, ceftazadime, colistin, gentamicin, imipenem,
meropenem, tigecycline and piperacillin/tazobactam. 57%
and 74% of the isolates were resistant to amikacin and
aztreonam respectively. While 60% and 69% of the isolates
were resistant to sulbactam/cefperazone and ceftazadime
respectively. A total of 14 (2%) isolates out of 705 clinical
isolates were also found to be resistant to Colistin.
Conclusion: Acinetobacter baumanii isolates show a
progressive trend of resistance to carbapenems and
colistin. Hence judicious usage of carbapenems and colistin
coupled with strict infection control protocols should be
implemented. Susceptibility testing for colistin resistance
should also be implemented in patients who have gone
under treatment with colistin methansulfonate for severe
infections.
Speaker Biography
Syed Bilal Tanvir holds an MD degree and a master’s in clinical
microbiology from Queen Mary, University of London. He completed
his postgraduate training in clinical microbiology at Barts and London
School of Medicine and Dentistry and the Blizard Institiute. He is
currently working in the position of a faculty member at Dar Al Uloom
University Hospital, Riyadh, Saudi Arabia. He has previously presented
his research in Jeddah, Karachi, Bahrain and London as well. His
research interests include surveillance of antimicrobial resistance,
systematic review and meta-analysis on newer antimicrobials and
surveillance of gram-negative nosocomial infections.
e:
Syed Bilal Tanvir et al., Microbiol Curr Res, Volume 3
ISSN: 2591-8036