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CANCER STEM CELLS AND
ONCOLOGY RESEARCH
11
th
International Conference on
Journal of Medical Oncology and Therapeutics
|
Volume 3
Sofiane Saada et al., J Med Oncl Ther 2018, Volume 3
A NEW LABEL FREE APPROACH FOR
CSC DETECTION ON GLIOBLASTOMA
MODEL
IN VITRO
Sofiane Saada
1
, Aurélie Lacroix
1
, Claire Dalmay
2
, Car-
ole Mélin
1
, Stéphanie Giraud
3
, Mathilde Cheray
1
, Gaelle
Bégaud
1
, Pierre Blondy
2
, Marie-Odile Jauberteau
1
, Barbara
Bessette
1
, Christophe Lautrette
3
, Arnaud Pothier
2
, Serge
Battu
1
and
Fabrice Lalloué
1
1
University of Limoges, Franc
2
Ester Technopole, France
C
ancer stem cells (CSCs) were a critical point in cancer making them
a central target of the different area of interest for new diagnosis,
therapeutic and theranostic approaches. The postulate of poor number of
CSCs in cell culture and the lack of real specific markers to well recognize
these cells conduct to establish new methods to isolate and characterize
them. Invent label free technique described in this study is able to provide a
great number of CSCs and finally deserve a CSCs signature and neutralize
them. This process is based on combine devices forming. Several culture
conditions allows an enrichment in CSCs. This enrichment was enhanced
using SdFFF cell sorting method. This method was coupled to a biosensor
to measure a specific electromagnetic (EM) signatures corresponding to
each condition obtained. A unique EM signature was also obtained for
CSCs opening the way to study these critical cells in glioblastoma and
more largely in cancer.
Sofianne Saada is currently working at the
Université de Limoges, Faculté de Médecine,
France.
sofiane.saada@unilim.frBIOGRAPHY