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CANCER STEM CELLS AND
ONCOLOGY RESEARCH
11
th
International Conference on
Journal of Medical Oncology and Therapeutics
|
Volume 3
C O Rodini et al., J Med Oncl Ther 2018, Volume 3
EVIDENCE FOR A MOLECULAR
SIGNATURE OF METASTATIC
POTENTIAL OF AN ORAL SQUAMOUS
CELL CARCINOMA CELL LINE
C O Rodini
1
, N M Lopes
1
, R A Alavarce
1
, T J Dionísio
1
, R C
Ortiz
1
, N G Amôr
1
, R F Buzo
1
and
E Graner
2
1
University of Sao Paulo, Brazil
2
University of Campinas, Brazil
R
ecent evidences show that there is a link between cancer stem cells
(CSC) and the process of epithelial-mesenchymal transition (EMT).
The purpose of the present study was to
in vitro
evaluate the combination
of the biological properties related to CSC and EMT phenotypes with
the invasive and metastatic behavior of the corresponding primary and
metastatic oral squamous cell carcinoma (OSCC) cell line SCC-9. To
accomplish this parental (SCC-9ZsGreen) andmetastatic (SCC-9ZsGreen
LN-1) OSCC cell lines, obtained after
in vivo
tumorigenesis assays, were
initially characterized regarding the ability of migration and invasion by
in vitro
scratch and 3D invasion assays, respectively. Further, qRT-PCR
was conducted to verify the differential expression levels of CSC (CD44,
BMI-1, ALDH-1 and p75NTR) and EMT (SNAIL1, TWIST1, AXL, vimentin,
E-cadherin and N-cadherin) markers in both tumor cell lines, using human
palate epithelial cells (HPEC) as control. The study provides evidence
of a CSC subpopulation within the metastatic cell line undergoing EMT
to acquire greater migratory and invasion capacities, depending on the
simultaneous overexpression of CD44, AXL, vimentin and N-cadherin,
associatedwith loss of E-cadherin. This can be considered as a “molecular
signature” of CSCs undergoing EMT (EMT-CSC) in OSCC, with potential
to be used clinically in the classification of tumors with higher or lower
metastatic potential, as well as to support new therapeutic strategies
against this neoplasm.
C O Rodini has completed her PhD at the age
of 30 years from University of São Paulo, Brazil.
She is an Assistant Professor of Biological Sci-
ences at Bauru School of Dentistry, University
of São Paulo, since 2010. She has 36 publica-
tions that have been cited over 200 times, and
her publication H-index is 11. She has been
responsible for two ongoing grants funded by
the Brazilian government (FAPESP). She is the
head of the research group “Cancer Stem Cells
in Head and Neck Cancer”, studying the role of
cancer stem cells and tumor microenvironment
in the process of invasion and metastasis of
oral squamous cell carcinoma.
carodini@usp.brBIOGRAPHY