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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Reproducibility of four identification methods of antibiotic-resistant
Mycobacterium tuberculosis
isolated from displaced and nondisplaced Iraqi patients with reference to QuantiFERON
Mohemid M Al-Jebouri and Burhan A Ali
University of Tikrit, Iraq
Background:
The first and major step in the diagnosis of TB
is its accurate and early detection. To fulfill this objective,
many methods have been developed and reported that
obtain early growth of
M. tuberculosis
. For exactly detection
of the TB cases, recently a novel polymerase chain reaction
(PCR) based diagnostic kit has been developed. It is based
on the nucleic acid amplification (NAA) of specific region of
Mycobacterium
DNA. QuantiFERON-TB test, (QFT) an
in vitro
diagnostic test that measures a constituent of cell-mediated
immune reactivity to M. tuberculosis was approved by Food
and Drug Administration (FDA) as an aid for identifying
Mycobacterium tuberculosis
infection.
Methodology:
In the current study, there were 50 patients
(18 displaced and 32 nondisplaced TB patients) and
40 healthy controls. The patient was examined for the
presence of TB utilizing QuantiFERON-TB Gold In-Tube assay,
polymerase chain reaction(PCR), AFB smear and TB culture.
Drug susceptibility of isolates to first-line anti-tuberculosis
drugs was performed using the proportion method on
Lowenstein Jensen medium (L J medium) within 2-4 weeks.
Results:
It was found that the frequency of positivity of
acid-fast stain, culture and QuantiFERON for displaced and
non-displaced patients was 36, 33.3 and 100 and 64, 66.7
and 100% respectively. The positivity towards polymerase
chain reaction for primers IS6110 and MPB64 for displaced
patients was 37.5 and 100% respectively, whereas for
nondisplaced patients was 14.3 and 100 % respectively too.
The present study revealed that 20 isolates out of 34 tested
were resistant to one or more of anti-tuberculosis drugs
tested which were isoniazid, streptomycin, rifampicin and
ethambutol. Statistically, there was a significant difference
between types of drug and frequency of resistance among
displaced and nondisplaced Iraqi patients (P˂0.05).
Conclusions:
The PCR test for the presence of primer
MPB64 and QuantiFERON test were 100% positive for
all mycobacterial isolates tested from displaced and
nondisplaced patients, whereas other identification tests
revealed variations in reproducibility. The present study
showed that all the mycobacterial isolates tested for
antimycobacterial drugs were resistant to at least one
antibiotic used and most of them were multiple-resistant.
Statistically, there was a significant difference between types
of drug and frequencies of resistance (P˂0.05).
Speaker Biography
Mohemid M Al-Jebouri is Professor at Department of Microbiology, College of
Medicine, University of Tikrit, Tikrit, Iraq. His research interest includes: Infectious
Diseases, Pharmaceutical Microbiology, Allergy and Immunity, Clinical Diagnosis and
Management by Laboratory Methods.
e:
profaljebouri@yahoo.com