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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
Detection of ER stress after infection of human macrophages by
Mycobacterium tuberculosis
Samuel Eguasi Inkabi
Linköping University, Sweden
P
ost infection, macrophages, the first cells in the lungs that
propel defense against pathogen invasion and play crucial
activity in the onset and maintenance of immune responses
against Mtb. The macrophages play this crucial defense role
by phagocytosis, which have the macrophages ‘‘eat’’ up the
Mtb
bacilli
. Macrophages therefore become infected with
mycobacteria and may undergo apoptosis (programmed cell
death) to destroy pathogens and prevent further spreading.
Apoptosis, which results in the elimination of Mtb can be
triggered by endoplasmic reticulum (ER) stress which is
the physiological or pathological processes that disturb
protein folding in the endoplasmic reticulum caused by
the phagocytosis of the Mtb
bacilli
by the macrophages.
The dysregulation of ER homeostasis can cause chronic
diseases in humans and it is crucial to study ER stress using
mammalian cells to understand ER-stress related diseases
such as Tuberculosis. Here, we studied the ER stress
induction and the extent of ER stress induction using human
monocytes derived macrophages (hMDMs). We used the
ER stress inducers tunicamycin and thapsigargin, and also
infecting the macrophages with different doses of Mtb and
analysing CHOP and ATF6-alpha expression by western blot.
This indicated that both inducers triggered CHOP activation,
that a low dose of Mtb suppressed the expression of these
ER-stress markers in most donors, and that infection with a
higher dose of Mtb stimulated expression of both markers
in 4 out of 6 donors. Alternatively, live microscopy was also
performed on Raw macrophages and 16HBE epithelial cells
after transfection with the ER stress plasmid sensor pEGFP-
XBP1dDBD-STOP-tagRFPt and stimulation with tunicamycin
and purified protein derivative of tuberculin (PPD). We
have here confirmed the detection of ER-stress in human
monocyte derived macrophages using positive inducers,
and shown that low doses of Mtb decreases induction of ER-
stress whereas, high dose of Mtb induces ER- stress
Speaker Biography
Samuel Eguasi Inkabi has completed his MSc in Medical Biology from Linkoping
University, Sweden. He also holds a Bachelor’s in Biochemistry from Kwame Nkrumah
University of Science and Technology, Ghana. His research focuses on infectious
diseases, cancer and avian genetics. He has co-authored a publication and authored
peer review papers in reputed journals.
e:
samin711@student.liu.se