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Microbiology: Current Research 2017 | Volume 1, Issue 2
Joint Conference
GLOBAL APPLIED MICROBIOLOGY CONFERENCE
MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017
Toronto, Canada
International Congress on
&
A stem bark extract from
Khaya grandifoliola
(
Meliaceae
) C DC. stimulates the chemotactic and
phagocytic activity of rat peritoneal macrophages exposed to CCl4
Florence Pare Ngoungoure
University of Yaoundé I, Cameroon
K
haya grandifoliola
is a plant species used in traditional
medicine in Cameroon for the treatment of many
diseases. This study determines the capacity of chloroform/
methanol (CH2Cl2/CH3OH) (1:1v/v) extract of stem bark
of the plant to boost the chemotactic and phagocytic
activities of rats macrophages in response to challenge
with
Saccharomyces cerevisiae
.
In vitro
study showed that
Khaya grandifoliola
extract (KGE) caused a non-dependent
concentration increase in NO production, reduced NBT dye
by 75% and enhanced the activity of lysosomal enzyme by
47% at 1 µg/ml. The results of the
in vivo
study shows that
after seven consecutive days of CCL4 exposure, the dose 100
mg/kg of body weight, KGE significantly (p< 0.05) increased
the activity of NADH oxidase (1.3 folds), lysosomal enzyme
(2 folds); production of H2O2 (2 folds) and NO (7 folds)
compared to control group. We conclude that KGE stimulates
the phagocytic activity of macrophages both
in vitro
and
in
vivo
making it a candidate substance for strengthening the
immune system
.
e:
florrary@yahoo.fr