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N o v e m b e r 2 1 - 2 2 , 2 0 1 8 | M a d r i d , S p a i n
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Analytical Chemistry 2018 Journal of Chemical Technology and Applications| Volume 2
ANALYTICAL CHEMISTRY
International Conference on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Gorgaslidze N & M Getia, J Chem Tech App 2018, Volume 2
DEVELOPMENT OF HPLC METHOD FOR
ESTIMATION OF BROMELAIN IN GEL
FORMULATION
At the TSMU Iovel Kutateladze Institute of Pharmacochemistry has devel-
oped Bromelain containing gel with fibrinolytic, antithrombotic, anti-tumor,
anti-edema and anti-inflammatory properties. The active compound of gel for-
mulation is complex of proteolytic enzymes – proteases derived from the stem
of Ananas comosus. Bromelain is being the chemical and biological marker of
the gel formulation. A simple and reliable HPLC method is developed for the
quantitative evaluation of the gel formulation using this compound.
The isocratic separation was achieved using an XDB C-18 (4.6 x 250 mm; 5
µkm). The UV detection is performed at 280 nm. All separations were real-
ized at 20°C.Mobile phase consisting of solvent A (acetonitrile) and solvent B
(0.1% formic acid in water) [10:90] at a flow rate of 1 ml/min.
The proposed HPLC method is linear in the range studied (r2> 0.9996) for all
the analytes. The method is precise with intra- and inter-day variations of less
than 1.68%. Precision, sensitivity and linearity are satisfactory in the range
studied. Accuracy 99,2±0,9%. The method was validated as per ICH guidelines
and can be adopted for the routine analysis of Bromelain in gel formulations.
Biography
Gorgaslidze N has completed her PhD at the age
of 37 years from Saint-Petersburg State Chemi-
cal-Pharmaceutical Academy, Russia. She is a di-
rector of TSMU Iovel Kutateladze Institute of Phar-
macochemistry and professor at the department
of Social and Clinical Pharmacy at Tbilisi State
Medical University. She has published more than
100 publications in reputed journals, the author of
4 books and 2 patens. She is a member of organiz-
ing committee of several international conferences
and meetings. She has more 40 years of teaching
experience at the Tbilisi State Medical University,
Georgia. She is founder of the Georgian Pharma-
ceutical Association (President 2002-2005) and
newspaper - “Pharmacy”. She is member of Scien-
tist and Young Pharmacists of Georgia. Nana Gor-
gaslidze has long timework experience in the Min-
istry of Health, Labor and Social Affairs of Georgia
in field of state control of quality medicinal and
pharmaceutical products and other departments
of the same ministry.
Gorgaslidze N and M Getia
TSMU Iovel Kutateladze Institute
of Pharmacochemistry, Georgia