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Archives of Industrial Biotechnology | Volume 2
May 14-15, 2018 | Montreal, Canada
World Yeast Congress
N
anoparticles are commercially used in everyday products
including zinc sunscreen and water resistant fabrics and
surfaces, but in the future they may be used in the targeted
treatment of cancer, printable monitoring systems and
affordable phones. Understanding the effects of nanoparticles
on biological organisms is crucial for the responsible use of
these technologies. We investigated the effects of silver (Ag)
and cadmium (CdSe/ZnS) nanoparticles on the budding
yeast
Saccharomyces cerevisiae
using growth assays, FUN-1 staining
for metabolic activity, RNAseq and RTPCR. Our growth assay
showed that Ag has an inhibitory effect with its concentrations
above 5µg/ml, whereas SdSe/ZnS had no effect on cell growth.
Interestingly, cells treatedwith 5µg/ml Ag showed nometabolic
defects. Hundreds of the same genes in both Ag and CdSe/
ZnS treated cells were differentially expressed according to
our transcriptome investigation, the majority of which are
responsible for ribosomal biogenesis and nucleotide binding.
Furthermore, we validated the RNAseq results using an RTPCR
assay. The resulting expression profile leads us to suspect
that Ag and CdSe/ZnS nanoparticle exposure creates a stress
environment in the cell.
Speaker Biography
Kyoungtae Kim is a Professor at Missouri State University in Springfield, MO. He
received his BA and MA in Biological Science at Kyungpook National University in Taegu,
Korea. He went on to obtain his PhD in Biology at Florida State University in Tallahassee,
Florida, and completed his Post-Doctoral at Washington University in St. Louis, Missouri,
where he studied Cell Biology and Physiology. He is now located at Missouri State
University where his research focuses on Diverse Cellular Processes including Endocytic
Pathway, Intracellular Trafficking of Proteins and Membranes, Membrane Organization,
Nanomaterial Traffic and Nanomaterial-Mediated Global Gene Expression Pattern
Changes.
e:
kkim@missouristate.eduKyoungtae Kim
Missouri State University, USA
Functional up regulation of ribosome biogenesis in yeast treated with silver or CdSe/
ZnS nanoparticles