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Journal of Clinical and Experimental Toxicology | Volume: 2
December 03-04, 2018 | Dubai, UAE
International Conference on
6
th
International Conference on
Toxicology, Clinical Toxicology & Pharmacology
Recycling & Waste Management
Joint Event
&
Impact of cadmium on the endocrine and exocrine sexual activity in the adult male and female wistar
rats: Determination of an apoptotic process
Raed Abdennabi
Laboratory of Pharmacognosy and Natural Products Chemistry, Greece
T
his study deals with the impact of chronic exposure to
cadmium on male and female’s fertility in rats. In fact, some
male and female rats are given distilled water for drinking (T:
controls), whereas the other ones are given distilled water
enriched with chloride cadmium, either 10 (C1 group) or
20 mg/l (C2 group) for 1, 5, 10, 15, 30, 45, 60 or 90 days. In
male rats, Cadmium, which induced a pathological change in
spermatogenesis, is observed by histological studies: arrest
of cell germ maturation, Then, an alteration of the structure
of the seminiferous tubes with blockage of spermatogenesis
(presence of male gametes without flagella or total absence
of spermatozoa and destruction of the sertoli cells, testified by
the presence of spans). Then, a decrease of the motility and the
number of spermatozoa at the end of the treatment indicates
the local cytotoxic effect of cadmium (Cd) on spermatogenesis,
inducing an apoptotic phenomenon revealed by borated
toluidine blue in the testicular cells, which affects the different
stages of spermatogenesis. Serum testosterone level is found to
be low at the beginning of the treatment, reaches a peak, then
returns to the control values and even exceeds them in spite of
the continuation of the treatment. It is therefore a hormonal
adaptation to this pollutant. In female rats, the practiced vaginal
smears revealed the oestrus phase in all the groups. Exposed
females are mated to control males, and fertility is assessed
later by counting the number of pregnancies. Fertility is found
to be reduced in females of C1 and C2 groups as compared to
control females (T group).
e:
raedabdennabi@gmail.comToxicology 2018 & Recycling 2018, Volume 2
DOI: 10.4066/2630-4570-C1-003