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academies
October 22-23, 2018 | Frankfurt, Germany
International Conference on
Robo t i c s a n d A u t oma t i o n
B iomater ial s and Nanomater ial s
Joint Event
&
Journal of Biomedical Research | Volume 29
Comparative assessment of various scaffolds for the construction of artificial tissues
Martin Heller
Johannes Gutenburg-Universiy of Mainz, Germany
In reconstructive surgery artificially generated soft tissue
equivalents are a powerful alternative to commonly used
autologous tissue transplants in order to cover bigger defects
after tumor resection or after trauma. However, the generation
of such tissue equivalents is complex and requires adequate
cell compatible scaffolds for successful cell cultivation. In
order to investigate the influence of various cell compatible
matrices on cell viability and differences in cellular morphology
in a complex co-culture of fibroblasts and epithelial cells, four
naturally derived different collagen matrices were analyzed in
a comparative study. From human buccal mucosa specimens,
fibroblasts and epithelial cells were cultured separately. In a
first step, primary fibroblasts were seeded on the four different
collagen matrices BioGuide® (BG), BioGuidePro (BGP) and
TissuFoil (TF) and small intestinal mucosa (SIS). The cellular
morphology on seeded matrices was analyzed by confocal
laser scan microscopy. The viability of the cells was quantified
by MTT assay. For co-culture, the primary buccal epithelial cells
were seeded on the opposite site of the fibroblasts covered
matrices. After 18 days of cultivation microsections were
analyzed using Masson-Goldner and immunohistochemical
staining (Cytokeratin 13, Tenascin, Collagen IV). In a co-culture
of fibroblasts and epithelial cells, BGP turned out to be the
most suitable matrix. Fibroblasts growing on BGP revealed the
greatest viability. Regarding mechanical characteristics such as
shrinkage, degradability and handling, BGP proved to be the
superior to the remaining matrices tested. Co-culture with
epithelial cells resulted in epidermal stratification, a developing
basement membrane. BPGmatrix is a promising biomaterial for
developing a full-thickness engineered buccal mucosa including
cell differentiation and maturation similar to the native tissue
when seeking new methods of urethral reconstruction.
Speaker Biography
Martin Heller has completed his PhD in Biology at the Max Planck Institute of Polymer
Research Mainz in 2013. Afterwards he worked as Postdoc at the University Medical
Center of Mainz and started to study Medicine in April 2014. His focus of research is the
modification of biomaterials in the context of artificially generated tissue equivalents in
complex multi-cultures of primary human cells.
e:
Martin Heller, Robotics & Biomaterials 2018, Volume 29
DOI: 10.4066/biomedicalresearch-C6-016