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7th International Conference on
Recycling and Waste Management
October 03-04, 2019 | Melbourne, Australia
Journal of Environmental Waste Management and Recycling | Volume: 02
Production and Characterization of cellulose from indigenous fungal strain NSF-2
using agro industrial waste and its use in waste paper recycling
Namita Singh and Anita Devi
Jambheshwer University of Science and Technology, India
T he study, present the production of cellulase using agro-
industrial waste, its purification and characterization. A
fungal strain NSF-2 isolated from the plant litter produce
high amount of cellulase. 15.59 fold purified enzyme
achieved specific activity 71.421 U/mg. Under the
optimum condition, Vmax and Km for the enzyme were 1.5
mg/ml/min and 3.2 mg/ml respectively. Purified cellulase
stable in non-ionic surfactant like TWEEN -20, Triton X-100
on the other hand salt like urea, EDTA and SDS inhibited
the enzyme activity up to 65%, 50% and 55% respectively.
The study also reveals that Hg2+ is a non-competitive
inhibitor of cellulase. The Vmax was decreasing with
increasing concentration of metal inhibitor. The purified
and crude cellulase produce by NSF-2 strain is well suitable
in industrial application such as pulp and paper for waste
paper recycling which also reduces the use of chemical
thus help in waste water management also.
Speaker Biography
Namita Singh completed her Masters and Doctorate degree inMicrobial
Biotechnology from Institute of Microbial Biotechnology, Barkatullah
University, Bhopal (India) in collaboration with Ben- Gurian University,
Israel. She is presently working as Professor at the Department of Bio
and Nano Technology, Guru Jambheshwar university of Science and
Technology, Hisar, Haryana, India. She has over 22 years of research and
17 years of PG teaching experience. She has guided 15 Ph.D., 96 .,
4 M.Tech. and 2 M.Phil. students till so far. Presently, 6 Ph.D. scholars
and 9 . students are pursuing research under her guidance. She
had received various prestigious fellowships such as UNESCO-American
Society For Microbiology, PDF by Jacob Bluestein Institute for Deserts
Ben-Gurion University of Negev sde Boker Campus, ISRAEL. She had
received EP Odum Gold Medal from International Society for ecological
communication in June 2009. She is a widely travelled person. She had
done several international academic visits in various countries such as
Israel, Japan, Germany, Canada, England, Dubai, Ukraine & Ethiopia.
She is also the member of various National and International scientific
societies of repute. She had coordinated over 30 national/ international
conference/ workshop/ seminars/ training programs. She has published
40 national and 60 international publications, 1 book, 3 manual, 123
sequence published on NCBI & 1 Patent. She is the Deputy Coordinator
of SAP DRS I & II at Department of Bio and Nano Technology, GJUS&T,
Hisar. She has completed eighteen national projects successfully and
currently working on 03 national and 01 international Project. She
has received several grants from national and international funding
agencies like DST, UGC, DBT, HSCST, MOEF Haryana etc. to support her
research activities.
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