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N o v e m b e r 2 3 - 2 4 , 2 0 1 8 | B a n g k o k , T h a i l a n d
Plant Science Congress 2018
Journal of Agricultural Science and Botany
ISSN: 2591-7897 | Volume 2
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
PLANT GENOMICS
AND PLANT SCIENCE
World Congress on
J Agric Sci Bot 2018, Volume 2 | DOI: 10.4066/2591-7897-C2-006
INTERACTION IN PLANT GROWTH REGULATOR AND PHYSIOLOGICAL
PROCESSES TO CONTROL PLANT REGENERATION AND
IN VITRO
DEVELOPMENT OF
TULBAGHIA SIMMLERI
Aloka Kumari
University of KwaZulu-Natal Pietermaritzburg, South Africa
T
he endogenous auxin and cytokinin contents of
in vitro
regenerated
Tulbaghia simmleri
maintained on applied plant growth
regulators in Murashige and Skoog (MS) mediumwere investigated using UHPLC-MS analysis. The highest number of shoots
(27.6 per leaf) were produced in MS medium supplemented with 2.5 µM thidiazuron. A higher number of these shoots were
rooted with 10 µM 6-(2-hydroxy-3-methylbenzylamino) purine (PI-55, cytokinin antagonist). Production of somatic embryos (SEs:
16.4 – 4.6, globular to cotyledonary stages) improved significantly with liquid MS medium containing 2.5 µM picloram, 2.5 µM
phloroglucinol (PG) and 1.5 µM gibberellic acid or 1.5 µM PI-55 and 1.0 µM trans-zeatin. SEs (torpedo and cotyledonary stages)
germinated (100%) in plant growth regulator free MS medium. The plantlets were acclimatized, and all survived in the green-
house. Higher levels of endogenous auxin, 2-oxindole-3-acetic acid (oxIAA, 371.52 pmol/g DW) and indole-3-acetylaspartate
(IAAsp, 141.56 pmol/g DW) were detected in shoots from PG treatments. The roots of garden-grown-mother plants possessed
the highest level of indole-3-acetic acid (IAA, 630.54 pmol/g DW) and oxIAA (515.26 pmol/g DW). Cytokinins [CKs: trans-zeatin-
O-glucoside (tZOG), cis-zeatin (cZ) and N6-isopentenyladenosine-5´-monophosphate (iPRMP)] levels were relatively high in
shoots and roots of plantlets
in vitro
. However, PI-55 treatments influenced the development of plantlets promoting a higher bio-
synthesis level of iPRMP (418.06 pmol/g DW in root) and cZRMP (904.61 pmol/g DW in roots and 1427.83 pmol/g DW in shoots).
The reported protocol highlights the significance of exogenous and endogenous hormonal effects on large-scale production of
in
vitro
plant development and improves the understanding of physiological processes of CK metabolism, signalling and transport
in
in vitro
development.