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O c t o b e r 1 5 - 1 6 , 2 0 1 8 | T o k y o , J a p a n
Obesity Congress 2018, Diabetes Congress 2018 & Vaccines Congress 2018
Note:
Page 19
Biomedical Research | ISSN: 0976-1683| Volume 29
2nd WORLD OBESITY CONGRESS
2nd WORLD VACCINES AND IMMUNOLOGY CONGRESS
&
&
DIABETES AND ENDOCRINOLOGY
International Conference on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Uraiwan Intamaso, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C5-012
CONSTRUCTION AND
CHARACTERIZATION OF YEAST
DISPLAY OF VIRAL CAPSID PROTEIN:
A PRELIMINARY IMPLICATION FOR
PRODUCTION OF ORAL VACCINE
AGAINST NERVOUS NECROSIS VIRUS
Nervous necrosis virus (NNV) causes viral nervous necrosis that often reach-
es higher than 99% mortality rate in hatchery-reared larvae and juveniles.
There are still no effective vaccines currently available for NNV. Yeast surface
display of capsid proteins of red-grouper-nervous- necrosis virus (RG-NNV)
was constructed aimed at developing an oral vaccine in fish. RG-NNV infection
in fingerlings or juveniles that showed clinical signs of abnormal swimming
patterns was proved by RT-PCR and DNA sequencing. The 2,100 bp of DNA
fragment containing RNA2 capsid protein of RG-NNV fused to AGα 1 of S cer-
evisiae in linearized pPIC9K vector was electroporated into P pastoris GS115.
Yeast auxotroph isolates were preliminary selected by histidine-producing abil-
ity and geneticin resistance. The recombinant yeasts were cultured in buffered
minimal glycerol-complex medium (BMGY) and induced with 0.5% methanol in
buffered minimal methanol complex medium (BMMY). Only 50% of the expres-
sion of the fusion proteins was detected by Western blot. Immunofluorescence
labeling confirmed the correct localization and the predicted tertiary structure
proposed the exposed conformation of the fusion protein on the cell wall. Op-
timization of protein expression is required for fully surface protein expression
before the evaluation of the possible use of the capsid protein displayed yeast
as an oral vaccine against RG-NNV infection.
Biography
Uraiwan Intamaso have completed her PhD from
Montana State University, USA. Currently, she is
an Associate Professor in Microbiology, Division
of Medical Technology, Faculty of Allied Health
Sciences, Burapha University, Thailand. Her scien-
tific fields mainly focus in Molecular Virology and
now research interests expand to an innovative
approach in viral detection and vaccines. She was
invited as a keynote speaker on the topic advance
and innovations to detect enteric viruses in sea-
food and a speaker on the topic the emergence of
uncommon genotypes of rotaviruses in Thailand
at the 7th International Conference on Agriculture,
Chemical, Biological and Environmental Sciences
on 22-24 May 2017 at Kuala Lumpur, Malaysia. She
enjoys solving scientific problems and shares re-
search knowledge with other scientists.
Uraiwan Intamaso
Burapha University, Thailand