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Journal of Nutrition and Human Health | Volume 3
May 23-24, 2019 | Vienna, Austria
Joint Event
2
nd
International Conference on
Gastroenterology and Digestive Disor
ders
17
th
International Conference on
Nutrition and Fitness
&
In vitro antioxidant activity of sesame milk fermentation in human Low-Density
Lipoprotein (LDL)
Ulyatu Fitrotin
1,2
, Pudji Hastuti
3
, Tyas Utami
3
and
Umar Santoso
3
1
Indonesia Agency for Agricultural Research and Development (IAARD), Indonesia
2
Assesment Institutes for Agricultural Technology of West Nusa Tenggara, Indonesia
3
Gadjah Mada University, Indonesia
S
esame milk contains sesaminol triglucoside that showed
higher antioxidant activity when it is hydrolyzed by
β-glucosidase. The objectives of this study were to determine
in vitro antioxidant activity of fermented sesame milk (FSM)
extract and sesame milk (SM) extract against DPPH and LDL
oxidation and to examine phenolic, sesaminol triglucoside
content and β-glucosidase activity. Antioxidant activity was
examined using DPPH and TBARS assay with LDL as the
oxidation substrate. Sesaminol triglucoside was identified
withHPLCdiodearraydetector andβ-glucosidaseactivitywas
determined by measuring hydrolysis rate of ρ-nitrophenyl-β-
D-glucopyranoside (ρNPG). After fermentation of sesame
milk with L.
plantarum
Dad 13, the β-glucosidase activity was
70.3±0.023 mU/ml fermented sesame milk, the sesaminol
triglucoside content of SM and FSM were 5.65 to 2.56
mg/100ml of sesamemilk, respectively, the phenolic content
of SM and FSM were 3.81±0.10 and 7.9±0.08 mg GAE/g dry
sesame seed, respectively, radical scavenging activity of SM
and FSM 18±0.64, and 45.5±0.37% respectively. Fermented
sesame milk inhibited human plasma LDL oxidation by 1.82
compared to unfermented sesame milk. This result related
to hydrolysis of sesaminol triglucoside by β-glucosidase
which was produced by L.
plantarum
Dad 13 that resulted in
sesaminol. This results suggest that fermented sesame milk
extract has in vitro antioxidant activity in human LDL better
than sesame milk extract.
Speaker Biography
Ulyatu Fitrotin is a researcher in Assesment Institutes for Agricultural
Technology of West Nusa Tenggara, Indonesia. She has completed her
PhD from food science doctoral program of Gadjah Mada University
Yogyakarta, Indonesia. Her research focuses on antioxidant activity and
food processing.
e:
ulyaelariefy@gmail.com