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Journal of Materials Science and Nanotechnology | Volume 2
allied
academies
October 29-30, 2018 | London, UK
Nanomaterials and Nanotechnology
International Conference on
A
microfluidic device based on ionic current sensing system
for high-throughput and practical single bacteria and
mammalian cell sizing was developed and furthermore,
discrimination of bacterial species and mammalian cell
deformability was achieved. The highly precise sizing system
basedonblockingioniccurrentatnarrowmicrochannelprovided
the information on antibiotic resistant strains of bacteria.
Deformability changes associated with passage of adipose
tissue-derived stem cells (ASCs) were also successfully detected
by the device without any chemical or biological modification.
The mechanical properties of a cell are extremely important
because changes in the mechanical properties are indicative of
diseases ranging from diabetes to malignant transformation.
Considering the heterogeneity within a population of cancer
cells and stem cells, a robust measurement system at the single
cell level is required in both research and clinical situations.
Recent developments inmicrofluidic devices have advanced the
throughput of mechanophenotyping measurements. However,
since most of these assay techniques essentially rely on optical
detection systems, the spatial resolution was limited to a few
μm in the xy plane and less in the z direction. We have proposed
the microfluidic device with two consecutive constrictions for
a single cell sizing and deformability measurements based on
blocking ion current. In thiswork, we validated themethodology
and expanded the application field to stem cell research.
Speaker Biography
Noritada Kaji is a professor of the Graduate School of Engineering at Kyushu University,
Japan. He obtained a bachelor’s degree in Pharmaceutical Sciences in 2000 and PhD
degree in 2004 from the University of Tokushima, Japan. In his PhD study, he developed
nanopillar chips that were a state-of-the-art µTAS combined with nano-fabricated
structures for DNA analysis. After his postdoctoral research, he started working as
an assistant professor of the Department of Applied Chemistry at Nagoya University
from February 2005 and promoted as an associate professor from November 2011. He
became a full professor of Department of Applied Chemistry at Kyushu University from
January 2018. His current research interests are mainly divided into the following parts;
Development of micro and nanofluidic for single molecule biophysics and molecular
biology, integration of whole biological processes on a single chip for systems biology.
e:
kaji@cstf.kyushu-u.ac.jpNoritada Kaji
Kyushu University, Japan
Micro and nano-sensing techniques for the diagnosis of bacteria and cells