microbes-infection-2017-posters-accepted-abstracts

4 / 8

alliedacademies
Microbiology: Current Research 2017
Volume 1 Issue 2 Microbes Infection 2017
Page 38
September 28-29, 2017 | London, UK
Microbes Infection
38th Annual congress on
PICOSECOND LASER SURFACE TEXTURING
OF STAINLESS STEEL AND TI-6AL-4V AS A
METHOD TO REDUCE THE ADHESION OF
BACTERIA
Fatema H Rajab1, Christopher M Liauw2, P Benson2, L Li1 and K A
Whitehead2
1The University of Manchester, UK
2Manchester Metropolitan University, Chester Street, UK
Biofilm formation and colonization is initiated by bacterial
attachment followed by bacterial adhesion and retention
on a surface. The buildup of biofilms may result in related
health problems in the medical field and potential biofouling
issues in industrial settings leading to increased economic
burden. The design and manufacture surfaces that prevent
bacterial attachment, retention and biofilm formation
through their physical structure and chemical properties
provides a potential solution to tackle such issues. Laser
surface texturing provides a crucial role for the production
of different antifouling surface patterns for use in a diverse
range of applications in different medical or industrial fields.
In the present work, a 1064 nm Nd:YVO4 Picosecond laser
was used to produce a range of textures on 316L stainless
steel (SS) and Ti substrates. Surface parameters were
determined; topography and roughness using a ZeGage
Optical Profiler and wettability using a contact angle analyzer
FTA 188. Escherichia coli (E. coli) attachment, adhesion and
retention assays on the laser textured SS and Ti surfaces were
investigated using three different assays (spray with wash,
spray and retention). Scanning electron microscopy was
used to determine the number of attached/adhered/retained
bacteria. Results showed that the Ra values and wettabilities
of the surfaces all increased when compared to the control
following laser treatment. This work demonstrated that on
all the surfaces, for all the assays, the number of adhesive
bacteria on the laser textured surfaces was reduced compared
to the untreated substrate.
Microbiology: Current Research 2017