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Microbiology: Current Research 2017
Volume 1 Issue 2
Notes:
Microbes Infection 2017
September 28-29, 2017 | London, UK
Microbes Infection
38
th
Annual congress on
Ebtisam Al-Ali, Microbiology: Current Research 2017
Phylogeonography and molecular
characterization of Tomato yellow leaf curl
virus in Kuwait
T
omato is one of the most important vegetables cultivated
in the world, and is a critical component of nutritional
security. Tomato crops in Kuwait are frequently infected with
viruses, causing considerable losses, and dramatic reduction
in crop production. A Begomovirus isolated from severely
diseased tomatoes collected over two- tomato growing seasons
in the main tomato-growing areas of Kuwait and characterised
at the molecular level, the complete genomic sequence
determined based on the genome structure, organization,
and phylogenetic analysis, the Begomovirus found to be a
strain of
Tomato yellow leaf curl virus
(TYLCV). Two isolates
that characterized in this study had 97% and 95% nucleotide
sequence identity, with previously characterized Kuwaiti
isolate. TYLCV-KISR and the highest sequence identity (95%)
was with that of TYLCV-Almeria (Spain) isolate. Phylogenetic
analysis showed that the three Kuwait isolates formed a
separate clade, which is suggestive of a different lineage from
known TYLCV sequences, and the isolate KW 15 could be a
novel variant of TYLCV.
Biography
E Al-Ali obtained her BSc in 1993 from Kuwait University, worked for Kuwait University
as Research Assistant, and then joined KISR on October 5, 1993 and led six projects.
She has published more than 25 papers in reputed journals and international
conferences. Her field of experience is in plant virus detection, primer design, cloning
and sequencing, ELISA, DNA Extraction, PCR Amplification, RCA Rolling Circle
Amplification, TYLCV detection on tomatoes. She was also trained twice in the
University of Wisconsin Madison under the supervision of Prof.Amy Charkowski as well
as University of Washington state under supervision of Prof. Hanu Pappu.
ebtisam_alali@hotmail.comEbtisam Al-Ali
Kuwait Institute for Scientific Research, Kuwait