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Microbiology: Current Research | Volume 3
May 20-21, 2019 | Vienna, Austria
Medical Microbiology
4
th
International Conference on
Virus hunting: A new variant of torque teno virus identified in Kawasaki disease
Yoshiro Nagao
Fukuoka Tokushukai Hospital, Japan
K
awasaki disease (KD) is an acute febrile illness which was
first reported in 1967. KD affects children, mainly of 0-10
years. Without prompt treatment, KD damages the arteries
in children's hearts, and can be potentially fatal. The number
of KD has been increasing rapidly in Japan, as well as in many
other countries. KD is possibly infectious, although its aetiology
has been unknown. To explore the aetiologic agent(s) of KD, we
enrolled 11 patients of KD and 22 matched control children.
The blood, faeces and nasopharyngeal aspirates were collected
from these 33 children. The DNA/RNA in these samples were
sequenced by a next generation high-throughput sequencer
(Illumina®). Subsequently, Livermore Metagenomic Analysis
Toolkit classified the DNA/RNA sequences into microbial
species. It was revealed that the samples from 2 of the 11 KD
patients contained large amounts of a new variant of torque
teno virus (TTV). In contrast, none of the control samples
contained this virus. TTV was first discovered by Nishizawa
et al in 1997, but it is yet to be elucidated whether TTV
causes a human illness. Our small-scale study showed that
TTV is a candidate for the aetiological agent(s) of KD. We
also estimated the sample size for a future large-scale study,
which would be necessary to determine the aetiology of KD.
The estimated sample size was very large.
Speaker Biography
Yoshiro Nagao earned BSc from University of Tokyo, MSc from London
School of Hygiene and Tropical Medicine and MD & PhD from Osaka
University. His clinical specialty is general internal medicine and pediatrics.
He worked in Fukushima (2011-2013) after the nuclear disaster and
served as Médecins Sans Frontières in South Sudan (2016). His research
interest is epidemiology of infectious diseases (e.g. malaria and dengue
hemorrhagic fever in Thailand and Indonesia) for which Cozzarelli Prize was
awarded from the National Academy of Sciences of the US. He is currently
working for Department of Pediatrics, Fukuoka Tokushukai Hospital.
e:
in_the_pacific214@yahoo.co.jpYoshiro Nagao, Microbiol Curr Res, Volume 3
DOI: 10.4066/2591-8036-C1-005