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Journal of Infectious Diseases and Medical Microbiology | Volume 3
November 11-12, 2019 | Singapore
International Conference on
3
rd
International Conference on
Pathology and Infectious Diseases
Pathology and Oncology Research
Joint Event
&
J Infectious Disease Med Microbiol
| Volume 3
Gastric cancer AnnexinA5 knockdown could suppress cell proliferation and
metastasis
Xin Li
Chengde Medical University, China
G
astric cancer is one of the most deadly disease around
the world. However, the mechanism of it is still unclear.
AnnexinA5 is one of the calcium-dependent phospholipid
binding protein which is involved in the cell membrane
dynamics and cell signal transduction. Whether AnnexinA5
play any role in the development of gastric cancer is still
unknown. In this study, RNA interfering was performed to
silence AnnexinA5 in gastric cancer cell lines MGC-803 and
MKN-45. Cell proliferation was detected by MTT method,
while cell metastasis was examined by Wound healing assay
and Transwell assay respectively. Western blotting was
employed to detect the expression of the EMT marker genes
and the key genes in the ERK signal pathway. Our results
indicated that AnnexinA5 acted as an oncogene suppressor
and could suppress cell proliferation and metastasis in gastric
cancer which might via ERK signal pathway.
e
:
hbcdlx@cdmc.edu.cn