global-hematology-2019-scientifictracks

Note:

Hematology and Blood Disorders | Volume 2

Page 21

July 25-26, 2019 | Amsterdam, Netherlands

OF EXCELLENCE

IN INTERNATIONAL

MEETINGS

YEARS

Global Hematology 2019

International Conference on

HEMATOLOGY AND

BONE MARROW TRANSPLANTATION

MACROCYTOSIS WITHOUT ANEMIA FOUND IN TWO SHIBA DOGS

Sakurako Neo, E Ogawa

and

H Ochiai

Azabu University, Japan

Background:

Compared with other dog breeds, erythrocytes in healthy Shiba dogs (Shibas) are typically mi-

crocytic. Approximately 10% of Shibas have erythrocytes with high K, low Na and high glutathione (GSH) con-

centrations, termed HK/HG cells. HK/HG cells have higher MCV (76.0 ± 5.1fL [mean ± SD]) than HK/LG cells (high

K, low Na and low GSH) at 65.0–68.0fL or LK cells (low K, high Na and low GSH) at 65.7 ± 4.1fL. Concentrations

of several amino acids, including glutamate (Glu), aspartate (Asp) and glutamine (Gln) in HK/HG cells are higher

than those in LK cells. Author’s encountered two atypically macrocytic Shibas without anemia.

Objectives:

This study aimed to investigate the physiological characteristics of erythrocytes in atypically mac-

rocytic Shibas (Macs).

Methods:

Blood samples were collected from two atypically macrocytic Shibas and three typical Shibas with

HK/HG cells (HK/HG) using EDTA or heparine as the anticoagulant. CBC, concentrations of K, Na, GSH and 20

amino acids including Glu, Asp and Gln within erythrocytes were compared.

Results:

MCV was higher in Macs than in HK/HG cells (Macs: 95.7 or 92.1fL, HK/HG 64.8–67.4fL). High K (Macs:

114 or 120mmol/L, HK/HG 119–138mmol/L), low Na (Macs: 8 or 7.2mmol/L, HK/HG: 8.9–11.5mmol/L) and high

GSH (Macs: 51.3 or 53umol/gHb, HK/HG 32.2–36.3umol/gHb) concentrations within erythrocytes, indicating

Macs’ erythrocytes to be HK/HG cells. Total amino acid concentrations were approximately 2.5 or 5-fold higher

in Macs compared to HK/HG cells.

Conclusion:

Higher amino acid concentrations may be attributable to macrocytosis in Shiba dogs.

Sakurako Neo et al., Hematol Blood Disord 2019, Volume 2

Sakurako Neo completed PhD from Azabu University, Japan in 2005, finished veterinary clinical pathology residency program in

2008 and became a Diploma of Veterinary Clinical Pathologist (ACVP) in 2010. Currently she is working as an Assistant Professor in

Veterinary School, Azabu University. Her research interest includes hematology (Specie specific hematology, eryptosis and micro-

particles) and coagulation.

BIOGRAPHY