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November 07-08, 2019 | Melbourne, Australia
Molecular Biology and Genetic Engineering
International Conference on
Journal of Research and Reports on Genetics | Volume 3
Molecular identification of filamentous fungi diversity in north coast beaches of Puerto
Rico
Echevarría L
Pontifical Catholic University of Puerto Rico, Puerto Rico
T
he Northern region has a great variety of beaches with
diverse microbial characteristics. Beach sands receive
direct contamination from the garbage generated by people,
which serves as nutrient for fungi growth. The objectives
of this investigation were to assess the filamentous fungi
diversity of four popular beaches; identify the genus and
species; and identify the taxonomic relationship between
the most abundant fungi. The beaches studied are located
in the towns of Vega Baja, Manatí, Barceloneta and Arecibo.
One sample of dry sand per month from three equidistant
points were acquired every month for a year in each beach.
The samples were homogenized according to dry (December-
April) and humid (May-November) seasons, for a total of four
composite samples per season. The DNA of each sample was
isolated and quantified; and, upon sequencing, evaluated by
metagenomic analysis with MG-RAST. There were 104 fungi
species identified by DNA sequencing analysis. The most
abundant were:
Aspergillus penicillioides, Aspergillus terreus,
Microascus sp., Arthrographis kalrae, Paramicrosporidium
sp., Dokmaia sp., Gliomastix polychroma
and
Aspergillus
sp
. The taxonomic analysis demonstrated that there is no
relationship in the genus of the most abundant species.
As a significant finding, 66 species of new registries were
identified, including
Malassezia restricta
,
Arthrographics
eremomycyces
, and
Cephaliophora tropica
. Not only
were many of the species pathogenic, several genus of
filamentous fungi have been previously isolated from
patients in nasal culture, and can cause eye, respiratory
and skin disease. The majority of these fungi use direct
contact and air transport as transmission vehicle to the host.
Speaker Biography
Echevarría L has completed her PhD. (Environmental Sciences), From Ana
G. Mendez University in 2016. She has published more than 3 papers in a
reputed journals and one book. She has been serving as an editorial board
of 3 journals. She is an associate professor of Environmental Sciences and
Biology department.
e:
lourdes_echevarria@pucpr.eduEchevarría
, J Res Rep Genet 2019, Volume 3
DOI: 10.35841/2591-7986-C1-003