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allied
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J Gastroenterol Dig Dis 2017 | Volume 2, Issue 3
World Gastroenterological &
Gastroenterology and Endoscopy
October 30-31, 2017 | Toronto, Canada
World Congress on
Effects of sex hormones on the toxicity and healing of gastric ulcer in rats treated with
Eugenia
punicifolia
, a medicinal plant from amazon region
Larissa Lucena Périco, Rodrigues, V P, Ohara R, Vilegas W, Rocha LRM, Santos C
and
Hiruma-Lima C A
UNESP, Brazil
Introduction:
Eugenia punicifolia
(Kunth) DC. (Myrtaceae),
popularly known as “pedra-ume-caá”, “pedra-ume”, “murta”
or “muta”, is a shrub largely distributed in the Amazon
region and Savanna biome. The leaves of this medicinal
plant are popularly used as a natural therapeutic agent to
treat inflammation, wounds and infections, among others
diseases.
Aim:
This study aims to evaluate the toxicity and the healing
effect of the hydroalcoholic extract obtained from the leaves
of E.
punicifolia
(HEEP).
Material & Methods:
The evaluation of the healing of
gastric ulcers induced by acetic acid in male and female
(intact and ovariectomized) Wistar rats, were performed
according to the method described by Okabe
1
modified
by Konturek
2
. HEEP (125 mg/kg - lower effective dose of
previous assays, dose-response curve), lansoprazole (30 mg/
kg) or vehicle (saline – 0.9% - 10 mL/kg) were administered
for the determination of the healing effects of the 14-day
subacute treatment. All treatments were done orally once a
day beginning one day after surgery. One day after the last
treatment, the rats were killed and the stomachs removed.
The gastric lesions were evaluated by pachymeter (mm
2
). To
evaluate subacute toxicity, body weight was recorded daily
throughout the experimental period, and the macroscopic
analyses and weight of vital organs were compared between
the three groups. Furthermore, blood (serum) was collected
for analysis of biochemical parameters, including glucose,
urea, creatinine, -glutamyl transpeptidase, aspartate
aminotransferase and alanine aminotransferase, were
measured using an automated biochemical analyzer. The
results were expressed as mean±standard error of the mean
of the parameters obtained and statistical significance was
determined by ANOVA followed by Dunnett’s test (minimum
level of significance:
p<
0.05).
Results & Discussion:
In the model of acetic acid, the
treatment with lansoprazole and HEEP were able to heal
significantly the gastric ulcer in male, intact females
and ovariectomized females after 14 consecutive days
(40.81% and 52.44% [male]; 84.21% and 85.22% [intact
females]; 49.40% and 65.47% [ovariectomized females],
respectively) of treatments when compared with control
group (vehicle). Intact females when compared to males,
showed a greater healing activity by treatment with HEEP
(1.44x) or lansoprazole (1.84x) (
p<
0.01). The comparison of
this same parameter in ovariectomized females and male
did not present significant difference. Moreover, after 14
days of treatment with HEEP, we did not observe any death,
changes in the body and organ weights, and in biochemical
parameters of serum in any of the groups.
Conclusion:
The oral treatment with hydro alcoholic extract
from
Eugenia punicifolia
leaves at dose of 125 mg/kg did not
cause toxicity after 14 consecutive days. The healed effect of
this extract against the highly damaging gastric agent (acetic
acid) is effective in male, intact females and ovariectomized
females rats and we can observe an interference of the
female sex hormones in the healing of the gastric lesion,
since lesion area of intact females was smaller than in males
and ovariectomized females.
Speaker Biography
Larissa Lucena Périco possess a bachelor’s degree in Pharmacy from the Faculdades
Adamantinenses Integradas (2010), a Master’s degree in Biological Sciences
(Pharmacology) from the Institute of Biosciences of Botucatu at the São Paulo State
University (UNESP) (2014). She is currently a doctoral student in Pharmacology and
Biotechnology at Institute of Biosciences of Botucatu (UNESP), where she works on the
following topics: Pharmacology of Natural Products, with an emphasis on medicinal
plants with antiulcerogenic, anti-inflammatory, antinociceptive and antidiarrheal
activity. Participates in the thematic project: “Standardized herbal medicines for the
treatment of chronic diseases”. During the master’s degree she worked with animal
models for gastroprotection. She currently works with animal models for the evaluation
of hormonal effects on gastric ulcer healing. Her current project is titled: The role of
the hydroalcoholic extract from the leaves of
Eugenia punicifolia
in experimental
peptic ulcer disease: Characterization of anti-inflammatory, healing and antiapoptotic
mechanisms of action.
e:
larissaperico@hotmail.com