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N o v e m b e r 2 6 - 2 7 , 2 0 1 8 | M a d r i d , S p a i n
&
&
BIOTECHNOLOGY
Euro Congress on
GENOMICS AND MOLECULAR BIOLOGY
International Conference on
CANCER SCIENCE AND THERAPY
Global Congress on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Euro Biotechnology 2018 & Genomics Congress 2018 & Cancer Congress 2018 Journal of RNA and Genomics | ISSN: 2591-7781 | Volume 14
Marwa Tantawy et al., J RNA Genomics 2018, Volume 14
PROFILING OF MIRNAS EXPRESSION IN
PEDIATRIC BRAIN TUMORS
Marwa Tantawy, Mariam G Elzayat, Dina Yehia and Hala Taha
Children’s Cancer Hospital, Egypt
Introduction: The understanding of pediatric CNS tumors biology is essen-
tial in the development of disease stratification and in development of less
toxic therapeutic agents as well as finding novel markers for early diagno-
sis. MicroRNAs are short 18–25 nucleotide small non-coding RNA molecules
regulate gene expression. Recent studies showed that miRNAs play a sig-
nificant role in brain tumor biology and may up-regulated or down-regulated
in malignancies, which referred their oncogenic or tumor suppressor effect.
MiRNA expression patterns have been linked to clinical outcomes, tumor reg-
ulation; such as tumor progression, cell growth, cell death and metastasis.
The identification of tumor specific miRNA signatures may assist in future in
the discovery of new biomarkers with diagnostic and prognostic utility. The
main objective of the present study is to detect the expression of different
miRNAs in different subtypes of pediatric CNS tumors to distinguish between
them in discovering biomarkers for early detection in addition to develop nov-
el therapies.
Methods: The expression level of 82 miRNAs were detected in 120 cases of
pediatric CNS tumors from fixed formalin paraffin embedded tissues (FFPE),
divided into four subtypes including; low grade glioma, high grade glioma,
ependymoma, and medulloblastoma using quantitative real time PCR (qRT-
PCR).
Results: Analysis of qRT-PCR data showed significant differences in miRNA
expression between tumor subtypes with P value < 0.05 and Low expression
of (miR-221, miR-9, and miR-181c/d) and over expression of miR-101, miR-
222, miR-139, miR-1827 and miR-34c) in medulloblastoma patients com-
pared to other subtypes. Low expression of miR-10a and overexpression in
(miR-10b, and miR-29a) in Ependymoma patients compared to other sub-
types. Low expression of miR-26a and over expression in (miR-19a/b, miR-
24, miR-27a, miR- 584 and miR-527) in low grade glioma patients compared
to other subtypes.
Conclusion: Micro RNAs are differentially expressed between different sub-
types of pediatric CNS tumors suggesting that they may play a significant role
in diagnosis to distinguish between different subtypes. A greater understand-
ing of aberrant miRNA expression in pediatric brain tumors may support in
the development of novel therapies. In addition, the characterization of tumor
specific miRNA signatures may play an important role in the discovery of bio-
markers with diagnostic or prognostic utility.
Marwa Tantawy from Pathology Department, Children’s
Cancer Hospital in Egypt. She completed her PhD in Ain
Shams University in 2016 and her masters in Immunolo-
gy and Parasitology at Cairo University in 2009.
BIOGRAPHY