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N o v e m b e r 2 6 - 2 7 , 2 0 1 8 | M a d r i d , S p a i n
&
&
BIOTECHNOLOGY
Euro Congress on
GENOMICS AND MOLECULAR BIOLOGY
International Conference on
CANCER SCIENCE AND THERAPY
Global Congress on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Euro Biotechnology 2018 & Genomics Congress 2018 & Cancer Congress 2018 Journal of RNA and Genomics | ISSN: 2591-7781 | Volume 14
Nina V Zaitseva et al., J RNA Genomics 2018, Volume 14
GENE POLYMORPHISM OF ESTROGEN
RECEPTOR AND APOLIPOPROTEIN E
ASSOCIATED WITH HYPERTENSION IN MEN
Nina V Zaitseva, Dolgikh O V, Alikina I N and Chelakova Yu A
Federal Scientific Center for Medical and Preventive Health Risk Management
Technologies, Russia
Background and Aim: The most common chronic disease among the adult
population of developed countries is hypertension (AH) that is the main risk
factor of disability and mortality. AH is characterized by a long latent period
and usually diagnosed at a late stage when end organ damage is non-revers-
ible. Therefore, the search for early AH predictors are relevant for medical
research. This study aims to examine the role of carrier state of polymorphic
loci of ESR1, АРОЕ4 and their expression product of ApoE as a factor of AH
in men.
Materials and Methods: 170 men with AH 1-2 degrees (observation group,
n=90) and without AH (comparison group, n=8) were surveyed. Genes poly-
morphism ESR1 (rs2228480) and APOE4 (rs429358) was studied using
PCR in real time with melting curve analysis. In the peripheral blood ApoE
level was examined using turbidimetry. Sample comparison was carried out
through dispersion analysis using the Kruskal-Wallis and the median tests.
Genotype distribution in the groups was estimated from the Hardy-Weinberg
equilibrium using the χ2 test in co-dominance and allelic inheritance models.
Results: In men with AH the minor allele of A ESR1 gene was found signifi-
cantly more often than in the comparison group (27.5% in the observation
group; 9.5% in the comparison group; χ2 =4.43; p=0.04). A reduction of ApoE
level in serum by men with AH associated with carriage of the TS genotype of
the APOE4 gene (Kruskal-Wallis test, p=0.04) was determined.
Conclusions: The data obtained certifies that carriage of the minor allele A of
ESR1 gene is associated with the development of AH in men. The reduction of
ApoE protein level in the serum of men with AH is associated with a polymor-
phism of АроЕ4 gene. The determined polymorphisms of candidate genes
are promising for use as additional markers of hereditary predisposition to
development of AH in middle aged men.
Nina V Zaitseva created and headed Perm Science and
Research Clinical Institute of Pediatric Ecopathology at
the present moment - Federal Scientific Center for Med-
ical and Preventive Health Risk Management Technol-
ogies Centre develops the modern methods of health
risk assessment associated with the impact of hetero-
geneous environmental factors and professionally deter-
mined as well as a system of evidences of harm to the
public health, as well as medical and preventive technol-
ogies to minimize the health risk. More than 30 research
works are carried out annually. More than 100 normative
and methodical documents have been developed for
practical implementation. She is the author of more than
850 publications and 50 patents of the RF for invention.
BIOGRAPHY