euro-biotechnology-genomics-congress-2018-keynote

4 / 8

N o v e m b e r 2 6 - 2 7 , 2 0 1 8 | M a d r i d , S p a i n
Note:
Page 17
Euro Biotechnology 2018 & Genomics Congress 2018 & Cancer Congress 2018 Journal of RNA and Genomics | ISSN: 2591-7781 | Volume 14
&
&
BIOTECHNOLOGY
Euro Congress on
GENOMICS AND MOLECULAR BIOLOGY
International Conference on
CANCER SCIENCE AND THERAPY
Global Congress on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Kirankumar S Mysore, J RNA Genomics 2018, Volume 14
INSERTION MUTAGENESIS IN MEDICAGO
TRUNCATULA AND ITS UTILIZATION TO
IDENTIFY NOVEL SOURCES OF RESISTANCE
AGAINST ASIAN SOYBEAN RUST
Retrotransposons, retrovirus like elements which encode proteins required
for their own replication and transposition can be used for insertional mu-
tagenesis. Tobacco retrotransposon, Tnt1, has been used to mutagenize and
tag the whole genome of a model legume Medicago truncatula . Tnt1 is very ac-
tive and transpose into on average, 25 different locations during M. truncatula
tissue culture. Mutations induced by Tnt1 insertion are stable during seed to
seed generation. We have generated over 20,000 independent Tnt1-containing
lines encompassing more than 500,000 insertion events. Over 400,000 Tnt1
flanking sequence tags (FSTs) have been recovered and a database has been
established. We have pooled genomic DNA from all the lines for customized
reverse genetic screening and the frequency of insert identification in this pool
for average sized gene is approximately 85%. The range and diversity of mu-
tant phenotypes obtained to date suggest that M. truncatula offers a great
opportunity to dissect symbiotic and developmental pathways for compre-
hensive understanding of legume biology. A forward genetics approach using
Tnt1 tagged M. truncatula lines has been established (Fig. 1) to identify genes
that confer non host resistance to Asian Soybean Rust pathogen Phakopso-
ra pachyrhizi. Several M. truncatula Tnt1 mutants with altered response to P.
pachyrhizi have been identified and being characterized. irg1 (inhibitor of rust
germ-tube differentation1) mutant inhibited pre-infection structure differenti-
ation of P. pachyrhizi and several other bio-trophic pathogens. IRG1 encodes
a Cys (2) His (2) zinc finger transcription factor, PALM1 that also controls dis-
sected leaf morphology in M. truncatula. Characterization of other mutants
will also be presented.
Biography
Kirankumar S Mysore is a Professor at the Noble
Research Institute. He also holds Adjunct Profes-
sorship at the Department of Entomology and
Plant Pathology, Oklahoma State University. He
received his Bachelor’s Degree in Agriculture at the
University of Agricultural Sciences, Bangalore, In-
dia. He did Master’s Degree in Horticulture at Clem-
son University and PhD in genetics at Purdue Uni-
versity in 1999. He did his Postdoctoral training at
the Boyce Thompson Institute for Plant Research,
Cornell University. His main research interests
center on molecular plant-microbe interactions.
Research approaches in his group include genet-
ics and genomics to better understand how plants
defend against pathogens. He has published over
180 papers and book chapters in international jour-
nals.
Kirankumar S Mysore
Noble Research Institute, USA