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Sarah T Idris et al., Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C6-018
INSILICO PREDICTION OF PEPTIDE BASED
VACCINE AGAINST FOWLPOX VIRUS (FPV)
Sarah T Idris, Shaza Salih, Mohamed Basheir, Anas Elhadi,
Sufyan Kamel, Khoubieb A Abd-elrahman, Ahmed Hamdi
and Mohammed A Hassan
Immunome research, Sudan
Fowlpox virus (FPV) is double stranded DNA virus and a member of
Poxviridae familywhich transmitted via aerosols and insect bite and causes
cutaneous and diphtheritic infection in poultry population. This study aimed
to design peptide vaccine by selecting all possible epitopes after analyzing
of all FPV140 protein sequence reported in NCBI database using insilico
approaches. After alignment of retrieved sequence the conserved region
applied into IEDB analysis tool to predict B and T cell epitopes, then testing
the affinity of predicted epitopes to bind to (BF2*2101) (BF2*0401) chicken
receptor for MHC1 molecule, peptides with low energy when docked against
receptor were suggested as epitopes based vaccine. Peptides (50 PPSPKP
55, 51 PSPKPL 56, 52 SPKPLP 57, 53 PKPLPK 58, 54 KPLPKS 59, 55 PLPKSK
60, 56 LPKSKQ 61 and 18 RPSSTV 23) were most potential B cell epitopes
while (110 YIMDNAEKL 118, 274 FYHRMYYPL 282, 278 MYYPLFSVF 286
231 YVVDNDRYV 239 and 317 LLSGVFLAY 325) docked epitopes suggested
to be T cell epitopes because of their good binding affinity especially this
overlapped one 110 YIMDNAEKL 118. This study concluded that those
predicted epitopes might use to produce good vaccine against FPV after
invitro and invivo studies to evaluate its efficiency.
Sarah T Idris has completed her master degree from
Sudan University of Science and Technology, Sudan.
She is medical laboratory specialist, with another
Master of Bioinformatics under process at University
of Bahri, Sudan. Working at National Medicines and
Poisons Board, Sudan.
BIOGRAPHY