cancer-summit-neuro-congress-2018-posters

Allied Journal of Medical Research

Volume 2

Page 44

allied

academies

CANCER THERAPY AND ONCOLOGY

NEUROLOGY AND BRAIN DISORDERS

International Conference on

J u n e 2 1 - 2 2 , 2 0 1 8 | O s a k a , J a p a n

Joint Event on

EPITOPEFINGERPRINTINGFORRECOGNITIONOFTHEPOLYCLONAL

SERUM AUTOANTIBODIES OF ALZHEIMER’S DISEASE

Lourena Costa, Lourena Emanuele Costa, Luiz Carlos de Oliveira-Júnior, Fabiana de Almeida Araújo

Santos, Luiz Ricardo Goulart

and

Carlos Ueira-Vieira

Federal University of Minas Gerais, UFMG – Brazil

utoantibodies (aAb) associated with Alzheimer’s disease (AD) have not been sufficiently characterized and their exact

involvement is undefined. The use of information technology and computerized analysis with phage display technology

was used, in the present research, to map the epitope of putative self-antigens in AD patients. A 12-mer random peptide

library, displayed on M13 phages, was screened using IgG from AD patients with two repetitions. Seventy-one peptides were

isolated; however, only 10 were positive using the Elisa assay technique (Elisa Index >1). The results showed that the epitope

regions of the immunoreactive peptides, identified by phage display analysis, were on the exposed surfaces of the proteins.

The putative antigens MAST1, Enah, MAO-A, X11/MINT1, HGF, SNX14, ARHGAP 11A, APC, and CENTG3, which have been

associated with AD or have functions in neural tissue, may indicate possible therapeutic targets.

Allied J Med Res 2018, Volume 2