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December 05-06, 2019 | Dubai, UAE
29
th
International Conference on
14
th
International Conference on
Nursing Education and Research
Cancer and Cancer Therapy
Joint Event
&
Journal of Medical Oncology and Therapeutics | Volume 4
The effect of melatonin and Vitamin D3 on the gene expression of P53 inMCF-7 Breast
Cancer cell line
Nora Saad Alkeraishan
King Saud university, Saudi Arabia
P
53 was identified as first tumor suppressor gene actively
involved in numerous cellular mechanisms such as
initiating DNA repair mechanisms, apoptosis and cell cycle
arrest.More than 50% of all human cancers have a mutated
nonfunctional p53 expression. Breast cancer(BC) is one of
the leading cause of mortality in females and mutated p53
is documented to be the causitive agent in only 20% of them.
However, mutation in p53 in BC results in more aggresive
form of cancer which is more resistant to the conventional
therapies.Recently multiple clinical trials suggested that the
combined use of melatonin and vitamin D3 can slow down
the growth of breast cancer cells. The genetic and molecular
mechanisms through which these compounds initiate the
cancerous cells to apoptosis or cell cycle arrest is still not
fully understood. This study aims to investigate the effect of
melatonin, vitamin D3 and their combined treatment on the
proliferation of breast cancer cell line MCF-7 by MTT assay.
Our results showed that melatonin,vitamin D3 and combined
effect of vitamin D3 + melatonin inhibit proliferation of these
cells by upregulating gene and protein expression of p53.
Speaker Biography
Nora Saad Alkeraishan is a Masters students at King Saud University, Riyadh
Saudi Arabia.She undertook this Research Project under direct supervision
of well known Scientist Dr. Samina Hyder Haq. Her intersting studies on
the effect of Melatonin and Vitamin D3 on Breast Cancer Cell line MCF-7
revealed that Melatonin could significantly upregulate p53 gene expression
in cultured cells and observed no direct relatioship of Vitamin D3 in
upregulating p53 gene expression. Currently she is involved in genotyping
the p53 to detect mutations present in the gene itself.
e:
nourasaadk@outlook.comJ Med Oncl Ther| Volume 4