+44-1518-081136Veterinary vaccines: Oriented approaches for intracellular and extracellular pathogens
21st World Congress and Exhibition on Vaccines, Vaccination & Immunization
November 09-10, 2017 Vienna, Austria
Angel Adrian Cataldi
National Institute of Agricultural Technology (INTA), Argentina
Posters & Accepted Abstracts : Virol Res J
Abstract:
Statement of the Problem: We work on two relevant cattle pathogens such as Mycobacterium bovis, agent of bovine TB and Escherichia coli O157:H7, the main member of the Enterohemorrhagic E. coli (EHEC) group, is the agent of uremic hemolytic syndrome. M. bovis and EHEC are both zoonotic pathogens. But while M. bovis is pathogenic to both cattle and humans, producing tuberculosis; EHEC do not provoke an illness in cattle (being cattle a reservoir) but causing a severe disease in human infants. In turn, M. bovis is an intracellular pathogen and EHEC is not an invasive pathogen. Methodology & Theoretical Orientation: We reasoned that to combat an intracellular pathogen, we need a live attenuated vaccine deleted in genes related to the virulence for that we designed a M. bovis wild type deleted in mce genes, additionally and for safety, another gene (phoP) was deleted. The vaccine probed to be effective in mice, guinea pig and cattle experimentally challenged. In the case of EHEC, we used recombinant proteins (rAgs) from the type 3 secretion systems and sometimes additional proteins. rAgs were emulsified in an adjuvant that promotes mucosal IgG response. Animals were challenged with an EHEC inoculum and a protection reducing the bacterial shedding and the number of excreting animals was observed. Conclusion & Significance: The rational design of vaccines is based in selecting the adequate approach to induce the adequate protective immune response for a given pathogen. In our case, results were promissory and prompt us to improve the current vaccine formulations to obtain efficient vaccines
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