Using Nano pore sequencing technology to screen for potential marine plasticdegrading microorganisms and relevant enzyme-encoding genes
3rd World Congress on Advances in Biotechnology
December 15, 2021 | Webinar
Matan Oren
Ariel University, Israel
Posters & Accepted Abstracts : J Biochem Biotech
Abstract:
The Nano pore Minion is a third generation highthroughput sequencing platform which is accessible to small-scale laboratories with a limited budget and/ or personnel. The Nano pore technology has several advantages over other next generation sequencing technologies, including extra-long reads, high speed, data availability in real-time and mobility of the sequencing device. On the other hand, the Nan pore sequencing average error rate is still relatively high. We used the Nano pore Minion in our lab in recent years for different research purposes, including whole genome sequencing of sea urchin single cells and testing approaches for the rapid identification of Cov-2 variants within incoming passengers at the Ben-Gurion airport. Our main research is currently focused on the identification of plastic-degrading microorganisms and enzyme-encoding genes that are related to the breakdown of different plastic polymers. Our results demonstrate that the Nano pore Minion technology is suitable for this purpose and after tuning of library preparation, sequencing and bioinformatics; it may outcompete other available sequencing platforms.
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