THE FOLDING MECHANISMS PREDICTION OF IG-LIKE BETA SANDWICH PROTEINS BASED ON INTER-RESIDUE AVERAGE DISTANCE STATISTICS METHODS

THE FOLDING MECHANISMS PREDICTION OF IG-LIKE BETA SANDWICH PROTEINS BASED ON INTER-RESIDUE AVERAGE DISTANCE STATISTICS METHODS

Joint Event on International Conference on STRUCTURAL BIOLOGY AND PROTEOMICS & International Conference on STD-AIDS AND INFECTIOUS DISEASES
September 03-04, 2018 | Bangkok ,Thailand

Panyavut Aumpuchin and Takeshi Kikuchi

Ritsumeikan University, Japan

Scientific Tracks Abstracts : J Genet Mol Biol

Abstract:

To understand the folding mechanism of a protein is one of the goals in bioinformatics study. Nowadays, it is enigmatic and difficult to extract the folding information from its amino acid sequence by using standard bioinformatics techniques or even experimental protocol which cost and time consuming. To overcome these problems, we aim to extract the initial folding unit for titin protein (Ig and fnIII domains) in the mean of inter-residue average distance statistics, average distance map (ADM) and contact frequency analysis (F-value). TI I27 and TNfn3 domains are represented for Ig-domain and fnIII-domain, respectively. Beta-strand two, three, five and six are significant for the initial folding processes of TI I27. On the other hands, the central strands of TNfn3 were predicted as a primary folding segment. Furthermore, known 3D structure and unknown 3D structure domains were investigated by structure or non-structure based multiple sequence alignment, respectively, to seek the conservation hydrophobic residue and predicted compact region through the evolution. Our results show well corresponded to experimental data, phi-value and protection factor of H-D exchange manner. It is confirming the significance of conserved hydrophobic residues near F-value peaks for structural stability by using hydrophobic packing. Again, our prediction methods could extract the folding mechanism by only its amino acid sequence.

Biography:

Panyavut Aumpuchin has completed his master’s degree in the field of Molecular Plant Pathology from Kasetsart University, Thailand. He is the PhD student of faculty of Life Sciences, Ritsumeikan University, Japan.

E-mail: a.panyavut@gmail.com

PDF HTML