Materials Science and Nanotechnology

Synthesis of biopolymer based platinum nanoparticles And 5-fluorouracil loaded Platinum nanoparticles and evaluation of their anti-tumor potential employing Potato Tumor Bioassay model

International Conference on Nanoscience & Technology
May 21-22, 2018 | New York, USA

Godugu Deepika

Osmania University, India

Posters & Accepted Abstracts : Mater Sci Nanotechnol

Abstract:

A facile and green synthesis of platinum nanoparticles (GKPtNP) using biopolymer, Gum Kondagogu (GK) as a reducing, capping and stabilizing agent was developed. The formation and stability of GKPtNP was confirmed by UV– visible spectroscopy, SEM-EDAX, TEM, XRD, Zeta potential analysis, FTIR, ICP-MS and stability studies. The formed GKPtNP are well dispersed, homogenous and showed a characteristic absorption peak at 201 nm, with a size of 2-4±0.50 nm and recorded negative zeta potential (−46.1 mV) indicating good stability. 5-fluorouracil (5FU), a known anticancer drug, was loaded into the synthesized GKPtNP, which leads to the development of a new combination of nanomedicine (5FU-GKPtNP). The in vitro drug release studies of 5FU-GKPtNP in pH 7.4 showed a sustained release profile over a period of 120 mins, and the hemolysis studies revealed that GKPtNP, 5FU and 5FU-GKPtNP were found to be blood compatible. A. tumefaciens induced in vitro potato tumor bioassay was employed for screening the anti-tumor potentials of GKPtNP, 5FU and 5FU-GKPtNP. The experimental results suggested, a complete tumor inhibition by 5FU-GKPtNP at a lower concentration than the GKPtNP and 5FU respectively. Further, the mechanism of antitumor activity was confirmed by their interactions with DNA using gel electrophoresis and UV-spectroscopic analysis. The electrophoresis results revealed that the 5FU-GKPtNP totally diminishes DNA and the UV-spectroscopic analysis showed hyperchromic effect with red shift indicating intercalation type of binding with DNA. Over all, the present study revealed that the combined exposure of the nanoformulation (5FUGKPtNP) resulted in the enhanced antitumor effect on A. tumefaciens induced potato tumor bioassay. e: deepikapharmaco@gmail.com

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