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Skin α-synuclein seeding activity as a biomarker of Parkinson's disease
3rd International conference on Parkinson's, Huntington's and Movement Disorders
September 27, 2021 | Webinar
Wen-Quan Zou, Zerui Wang*, Katelyn Becker*, Vincenzo Donadio*, Jue Yuan, Alex Incensi, Christina D. Orrú4, Curtis Tatsuoka, Rocco Liguori, Masih Rezaee, Brian Appleby, Steven A. Gunzler, Byron Caughey, Maria E. Jimenez-Capdeville, Li Cui, Shu G. Chen, Jiyan Ma
Case Western Reserve University, USA
Van Andel Institute, USA
IRCCS Institute of Neurological Sciences of Bologna, Italy
Laboratory of Persistent Viral Diseases, USA
University Hospitals of Cleveland, USA
University of San Luis Potosi, Mexico
The First Hospital of Jilin University, China
National Center for Regenerative Medicine, USA
Scientific Tracks Abstracts : Cogn Neurosci J
Abstract:
The pathological alpha-synuclein (αsynp) deposited in the brain is the hallmark of Parkinson’s disease (PD). Currently, definitive PD diagnosis often requires the detection of αsynp-containing Lewy bodies in brain tissue. Using the highly sensitive real-time quakinginduced conversion (RT-quic) and protein misfolding cyclic amplification (PMCA) assays, we observed significantly higher seeding activity of αsynp in skin tissue obtained at autopsy or by biopsy from PD patients than those obtained from non-PD controls. With the neuropathologicallyconfirmed cases, RT-quic revealed that sensitivity was 90% in abdominal and 100% scalp skin samples while both types of tissues had 100% specificity; PMCA revealed 83% sensitivity and 100% specificity with abdominal tissues. With the posterior neck skin biopsy tissues from clinicallydiagnosed PD and non-PD cases, RT-quic exhibited 93% sensitivity and 100% specificity, respectively. Our results provide proof-of-concept that the skin αsynp-seeding activity may be a useful biomarker for antemortem diagnoses of PD.
Biography:
Wen-Quan Zou received his medical degree from Jiangxi Medical College, his M.Sc. from Tongji Medical University, and his PhD. from Shanghai Medical University. He has practiced Internal Medicine and Nephrology for years in Nanchang and Shanghai, China, as both a physician and an attending physician, respectively. His postdoctoral work in neurodegenerative diseases, with a concentration in prion diseases, was done in the Department of Pathology at the Case Western Reserve University and at the Centre for Research in Neurodegenerative diseases at University of Toronto. Currently, Dr. Wen-Quan Zou is a Professor of Pathology with tenure and Associate Director of the National Prion Disease Pathology surveillance center at the Case Western Reserve University School of Medicine.
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