Reversed-phase UPLC-MS/MS analysis of serum reverse T3 (rT3) for clinical research

Reversed-phase UPLC-MS/MS analysis of serum reverse T3 (rT3) for clinical research

4^th Global Conference on Cancer Science and therapy & 9^th World Summit Virology, Microbiology & Infectious Disease & 6^th International Conference on Biomedical Biopharma and Clinical Research
October 11, 2022 | Webinar

Angelina I Nikitkina*, Elena S Simenel and Alexander N Nizhnik

ArhiMed Clinique for New Medical Technologies, Russia

Scientific Tracks Abstracts : Biomed Res

Abstract:

Reverse T3 (3,3',5'-triiodothyronine or rT3) is the third most abundant iodothyronine circulating in human blood and is produced by the inner ring deiodination of the pro-hormone thyroxine (T4) 80% of T3, and 95% to 98% of all reverse T3, is derived from peripheral conversion of T4 through deiodination. Both T3 and reverse T3 can shed more iodine atoms, forming in turn various isomers of T2, T1, and ultimately T0. Unlike the more abundant and active metabolite T3, the measurement of serum rT3 is yet to find a routine clinical application. Elevated level of rT3 associated with non-thyroidal illness syndrome (NTIS), stress, liver disease, inflammation, depression, and malignant tumors. rT3 is often analyzed by using clinical routine testing methods based on immunoassay (ECLIA and ELISA) which show limited sensitivity. Thus, a reversed-phase analytically sensitive with excellent linearity and precision, and minimal matrix effects UPLC-MS/ MS method was developed that employs a SPE procedure and combines it with reversed-phase chromatography using the Waters Atlantis T3 HPLC Column. The use of mixedmode anion exchange solid-phase extraction (SPE) plates, in combination with the unique retention and selectivity of the Waters Atlantis T3 HPLC Column and Shimadzu LCMS-8060, results in a rapid, fast and simple analysis of serum T4, T3, rT3 and T2.

Biography:

Angelina I Nikitkina has been working for Clinical Mass Spectrometry Laboratory of Clinique for New Medical Technologies ‘ArhiMed’, where she performs development, optimization and validation of a broad range of qualitative and quantitative (LC-MS, GC-MS) methods such as: quantification of vitamin D metabolites, amino acids, steroid hormones, melatonin, serotonin, thyroid hormones, catecholamines and their metabolites in biological samples (urine, plasma, serum, and saliva).

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