Prevalence of blood culture contamination in the collection of hematopoietic progenitor cells and blood components

Prevalence of blood culture contamination in the collection of hematopoietic progenitor cells and blood components

6^th Global Summit on Allergy and Immunology & 6^th International Conference on Clinical Hematology & Transfusion Medicine & 4^th World Congress on Infection Prevention and Control & 2^nd European Congress on Virology and Immunology
April 29, 2022 | Joint Webinar

Yair Omar Chavez Estrada*, Dalila Marisol Alvarado Navarro, Ana Karen Hernandez Navarro, Berenic Ake Uc, Víctor Gomez Lopez, Rosario Salazar Riojas, David Gomez Almaguer and Cesar Homero Gutierrez Aguirre

Mexican Society of Cell Therapy and Bone Marrow Transplantation, Mexico

Posters & Accepted Abstracts : Microbiology: Current Research

Abstract:

One of the risks present in patients receiving transfusion of hemocomponents or hematopoietic progenitor cells (HPC) during autologous or allogeneic transplantation is the administration of a product with bacterial contamination, increasing the possibility of an adverse event related to the transfusion, however, contamination can occur during the process, from the collection process to its infusion. In determining the prevalence of blood culture contamination in the collection and procedures performed for each blood component. The true detection of a positive culture represents a challenge between the timing of the result and the identification of true pathogens and contaminants. Through a retrospective study, we analyzed the results of blood cultures performed from 2013 to 2020, including collections of HPCs from mobilized peripheral blood (MPBS) and bone marrow (BM); and blood components (platelets, erythrocytes, and plasma) obtained by apheresis using cell separators. Each blood culture. Fourteen species of contaminating microorganisms were identified, with a greater predominance of Staphylococcus epidermidis and Micrococcus spp representing 37% and 11% among the other microorganisms identified. Acinetobacter spp. 7.4% (2/27), Clostridium spp. 3.7% (1/27) and Salmonella spp. 3.7% (1/27) were also reported. The inferences of contamination in the blood culture results is lower than the described ranges, considering it the reference standard of contamination more rigorous than other institutions <1.4%.