Phytochemical composition and biological properties of a hydroalcoholic extract obtained from the aerial parts of Matthiola incana (L.) R. Br. (Brassicaceae) growing in Sicily

doi:10.4066/2591-7897-C1-002

Phytochemical composition and biological properties of a hydroalcoholic extract obtained from the aerial parts of Matthiola incana (L.) R. Br. (Brassicaceae) growing in Sicily

Joint Event on International Conference on Plant Science & Natural Products,Medicinal Plants and Traditional Medicines
November 15-16, 2018 | Paris, France

Maria Fernanda Taviano, Emilia Cavo, Andreana Marino, Salvatore Ragusa, Paola Dugo, Francesco Cacciola, Pierangela Irrera, Concetta Condurso, Maria Merlino and Natalizia Miceli

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Italy Foundation Prof. Antonio Imbesi, University of Messina, Italy Magna Graecia University of Catanzaro, Italy Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Italy Department of Veterinary Sciences, University of Messina, Italy

Scientific Tracks Abstracts : Plant science & Natural Medicine

DOI: 10.4066/2591-7897-C1-002

Abstract:

In continuation of our studies on species belonging to the Brassicaceae family growing in Sicily (Italy), this work aimed to characterize the phenolic profile and the volatile constituents and to evaluate some biological activities of a hydroalcoholic extract (80% methanol) obtained from the aerial parts (leaves and flower buds) of Matthiola incana (L.) R. Br. grown wild around Capo D’Orlando (Messina, Sicily) The HPLC-PDA/ ESI-MS analysis led to the identification of twelve phenolic compounds, two phenolic acid derivatives (5.46 mg/g extract) and ten flavonoids (155.85 mg/g extract), being luteolinglucoside the most abundant component (57.07 mg/g ± 0.87% RSD). By SPME/GC-MS fifty-one volatile constituents were fully characterized, and (Z)-9-Octadecen-1-ol turned out to be the most abundant one (24.35%). The antioxidant potential of M. incana extract was evaluated by in vitro methods based on different approaches and mechanisms: DPPH, reducing power, and ferrous ions chelating activity assays. The extract exhibited mild activity both in the DPPH and reducing power assays, whereas it was found to possess good chelating properties, reaching approximately 90% activity at the highest tested dose (2 mg/mL). In order to investigate the antioxidant efficacy of M. incana extract in a biological setting, the ability to protect bacterial growth and survival from the oxidative stress induced by hydrogen peroxide (H2O2) was evaluated on Escherichia coli. The extract displayed protective effect against H2O2-induced oxidative damage. The antimicrobial activity of M. incana extract against selected bacteria and yeasts were assayed by standard methods. The extract didn’t show activity against the tested strains (MICs > 500 μg/mL). Finally, the potential toxicity was investigated using Artemia salina lethality bioassay. The median lethal concentration value indicated that the extract did not display any toxicity against brine shrimps (LC50 > 1000 μg/mL).

Biography:

Maria Fernanda Taviano obtained the following academic qualifications at the Faculty of Pharmacy of the University of Messina (Italy). Specialization degree in Pharmacognosy; PhD in Pharmacognosy; 1st level Master degree in Applied Biotechnology. She has been research grant holder for a period of four years. Currently, she is RTD-A Researcher (SSD BIO/15 - Pharmaceutical Biology) at the Department of Chemical, Biological, Pharmaceutical and Environmental Sciences of the University of Messina. She is Editorial Board Member of the scientific international journal “Pharmacognosy Magazine”. Her scientific research activity is mainly focused on the phytochemical study and the evaluation of “in vitro” and “in vivo” biological properties of active products from plant sources, obtainable also by means of “in vitro” culture systems.

E-mail: mtaviano@unime.it

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