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Novel detection protocol for radical scavenging and antioxidant activity of lipophilic antioxidants
8th International Conference on Nutrition, Food Science and Technology
December 09-10, 2019 | Dubai, UAE
Haue Md Azizul
Free University of Bozen-Bolzano, Italy
Posters & Accepted Abstracts : J Food Sci Nutr
Abstract:
Statement of the problem: Lipophilic antioxidants are an
important class of chemical species of natural antioxidants
that can increase the oxidative stability of food matrices.
Common methods to determine the antioxidant activity
or oxidative stress of lipophilic antioxidants require time
consuming protocols, although those are not vigorously
reliable. This work proposes a fast, simple and direct method
based on cyclic voltammetry to monitor oxidation in lipid
samples.
Methodology & Theoretical Orientation: The oxidative
stress during the reaction of AIBN (2, 2ยด-azobis
(20methylpropionitrile)) with lipid soluble antioxidants,
such asα-tocopherol, catechin, retinyl acetate, caffeic acid
and 3-hydxoxytyrosol was evaluated. 1-propanol was used
as a unique solvent, which allowed direct dissolution of a
wide range of lipid soluble redox species. Electron transfer
(ET) capacity was evaluated by the peak current (ip) and
peak potential (E). The kinetic rate of the reactions between
laboratory antioxidants and AIBN were measured at 60
°C. Finally, same procedure was also applied to measure
the antioxidant activity and oxidative stress of different
commercial edible oils: extra virgin olive oil (EVOO), virgin
olive oil (VOO) and, olive by product; sansa olive oil (SOO).
Findings: The methods demonstrated that antioxidant activity
was positively correlated with increased concentrations
among the laboratory antioxidants and EVOO, VOO and
SOO samples. On the other hand, oxidative stresses were
negatively correlated with the duration of reaction periods.
Conclusion & Significance: This method can be the alternative
of traditional methods to test lipid soluble antioxidants in lipid
matrices rapidly and straight.
Biography:
E-mail:
mdazizul.haque@natec.unibz.itPDF HTML