+441518081136Mitochondrial energy from omega-3 protects immunity and cognition of mild Cognitive Impairment patients beyond Cholinesterase inhibitors
13th International Conference on Alzheimers Disease and Dementia
November 25-26, 2019 | Frankfurt, Germany
Milan Fiala
UCLA, USA
Posters & Accepted Abstracts : J Psychol Cognition
Abstract:
Background: The innate immune system clears amyloid-β
(Aβ) by phagocytosis but fails in Mild cognitive impairment
patients (MCI) (reversibly) and in Alzheimer disease
patients (AD) (irreversibly). Omega-3 fatty acids (omega-3),
vitamin 1,25D3, and curcuminoids repair AD patients’
phagocytic and transcriptional defects.
Objectives: Immune and cognitive effects of
supplementation by a fish-derived lipid emulsion with
omega-3 protected against oxidation.
Method: We measured Aβ phagocytosis by the flow
cytometric Aβ blood test. We investigated the effects of
omega-3 on mitochondrial respiration and glycolysis in
immune cells using the Seahorse XF96 Extracellular Flux
Analyzer (Agilent). We performed RNA-seq of macrophages
using Illumina HiSeq 4000, aligned reads to the UCSC hg19
reference genome and obtained read counts using HT-Seq.
Result: Omega-3-supplemented MCI patients (MMSE >19)
maintained cognitive status close to baseline 1.7 to 5.1
years beyond cholinesterase inhibitors. Omega-3 had no
significant effects in patients with established Alzheimer
– type dementia (MMSE < 19) or Lewy body disease.
Omega-3 treatment of macrophages in vitro increased
transcription of enzymes for glycolytic and ox-phos
energies. Omega-3 increased basal oxygen consumption
rate (OCR), ATP-linked OCR, and OCAR/ECAR ratio in
peripheral blood mononuclear cells (PBMC’s). In omega-3
supplemented subjects, Aβ phagocytosis was active even
when glycolysis was inhibited by iodoacetate.
Conclusion: In a pilot study, cell signaling and increased
energy from a fish-derived emulsion of omega-3
recovered the immune functions of MCI through increased
mitochondrial energy and unfolded protein response. As
the changes in immune and cognitive functions in MCI
patients were correlated (r=0.77), the immune system may
have a disease-modifying role in some MCI patients.
Biography:
E-mail:
fiala@mednet.ucla.eduPDF HTML