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MIP nanoparticles in diagnostics and bioimaging
7th World Congress on Chemistry
November 13-15, 2017 Athens, Greece
Sergey A. Piletsky
University of Leicester, United Kingdom
Posters & Accepted Abstracts : J Biotech and Phyto
Abstract:
Two years ago we have made a major breakthrough in MIP technology developing solid-phase approach for preparation of soluble molecularly imprinted nanoparticles (nanoMIPs) with exquisite affinity and selectivity for their templates1. The success came from combining controlled radical polymerisation2 with an affinity separation step performed on surface-immobilised template3. This approach represents the state-of-the-art in nanoMIP synthesis: not only are soluble particles with defined size (20-200 nm) and a narrow size distribution produced in one hour, they possess subnanomolar dissociation constants for their respective targets, they can be easily functionalised with fluorescent, electrochemical or magnetic labels, and the immobilised template can be re used. High affinity nanoMIPs were made for small molecules, proteins, membrane proteins and virus particles4. The main practical niches for application of synthesised nanoMIPs are diagnostics, imaging and drug delivery. Particularly exciting is an opportunity to use MIP sensors in companion diagnostics. Members of our team have used nanoMIPs successfully as a replacement for antibodies in ELISA-type assays, electrochemical and optical sensors5. The exciting examples of our work with in vivo application potential are targeting membrane receptors, enzymes and quorum sensing molecules6. Very encouraging facts that enables practical applications of MIPs in vivo are lack of polymer toxicity, ability of nanoMIPs to penetrate into cells and to pass blood barrier. Current paper discusses challenges and opportunities that are faced by MIP technology in the light of these developments.
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