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Investigation of the molecular mechanisms underlying the antiatherogenic actions of kaempferol in human THP-1 macrophages
6th International Conference on CARDIOLOGY HEART DISEASE AND HEART FAILURE
September 19, 2022 | Webinar
Maha Majeed Ayoub
King Abdulaziz University, Saudi Arabia
Scientific Tracks Abstracts : Curr Trend Cardiol
Abstract:
The major cause of mortality worldwide is cardiovascular disease (CVD) (WHO, 2015). Atherosclerosis, hardening and narrowing of arteries, caused by accumulation of fatty acids and lipids (cholesterol plaques) is a main reason of stroke, myocardial infarction and angina. Present therapies basically use statins like β-Hydroxy β-methylglutaryl-CoA for cardiovascular disease showed less than 70% efficacy and multiple side effects. To evaluate the impact of kaempferol, a natural medication, against an atherosclerotic cell model, we undertook an in vitro investigation. The researchers used cytotoxicity assays, Boyden chamber invasion assays and quantitative real-time PCR. Affymetrix microarrays were used to profile the entire transcriptome of kaempferol-treated cell lines and Partek Genome Suite was used to interpret the results. THP-1 macrophages were not cytotoxic to kaempferol. In comparison to the control, kaempferol reduced monocyte migration mediated by monocyte chemotactic protein 1 (MCP-1) by 80%. MCP-1 (73.71-fold) and intercellular adhesion molecule 1 (ICAM-1, 2.47-fold) expression were both reduced in kaempferol-treated cells, according to qPCR results. For IFN-γ and IFN-γ + kaempferol-treated cells, we found 295 and 168 differentially expressed genes (DEGs), respectively. According to DEG pathway analysis, kaempferol exhibits anti- atherosclerosis and anti-inflammatory characteristics. Kaempferol is an effective and safe therapy for atherosclerosis.
References :
1. Lusis, AJ. Atherosclerosis. Nature 2002, 407, 233-241.
2. McLaren, J.; Michael, D.; Ashlin, T.; Ramji, D. Cytokines, macrophage lipid metabolism and foam cells: Implications for cardiovascular disease therapy. Prog. Lipid Res. 2011, 50(4), 331- 47.
3. Ramji, D.; Davies, T. Cytokines in atherosclerosis: Key players in all stages of disease and promising therapeutic targets. Cytokine Growth Factor Rev. 2015, 26(6), 673–85.
Biography:
Maha Majeed Ayoub is in cell culture unit and experimental biochemistry unit, King Fahd medical research centre, King Abdulaziz university, Jeddah 21589, Saudi Arabia.
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