+44-7360-538437In vitro differentiation of mesenchymal stem cells from human umbilical cord Wharton’s jelly into functioning hepatocytes
Joint Event On World Congress on Tissue Engineering, Stem Cells and Regenerative Medicine & International Conference on Cell and Gene Therapy
March 14-15, 2019 | London, UK
Mervat Dawood
Mansoura University, Egypt
Posters & Accepted Abstracts : Biomed Res
DOI: 10.4066/biomedicalresearch-C1-026
Abstract:
Back ground: The human umbilical cord (UC) is non-invasive,
primitive and abundant sources of mesenchymal stromal cells
(MSCs) that have increasingly. Liver disease is a major cause of
mortality and morbidity in Egypt. There are many inflammatory
liver conditions for which treatments are not effective and
often such patients will progress to end-stage liver disease and
require liver transplantation. To prevent progression to endstage
liver disease, mesenchymal stromal cell (MSC) therapies
have been considered and shown to have potential in such liver
diseases.
Objectives: The aim of our study was to investigate the in vitro
differentiation of human umbilical cord Whartonâs jelly (HUMSC)
into hepatocyte lineage.
Materials & methods: Human umbilical cord Whartonâs jelly
(WJ) were separated by mixed explant & enzymatic method
by use of trypsin. The time required for the primary culture
range from 10-14 days. The isolated cells were characterized
for expression of MSC-specific markers such as CD73, CD90
and CD105 & CD45. Also cells were counted by automated cell
counter for stem cells (showing count, viability, cluster cells).
After passage 4, the isolated cells induced to differentiate into
hepatocyte-like cells by incubation in basal media with cocktail
hepatocyte growth factors for 20 days.
Results: In vitro functional characterization of hepatocyte
detectable by PAS staining for glycogen and immunofluorescent
staining for albumin by anti-human albumin with FITC stain.
Conclusion: HU-MSC can differentiate into functional
hepatocyte like cells & serve as a cell source for tissue
engineering and cell therapy for hepatic tissues.
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