HIBISCUS FLOWERS AND OLIVE LEAVES EXTRACTS-BASED FORMULATION IN NEURODEGENERATION: A NETWORK-BASED APPROACH

HIBISCUS FLOWERS AND OLIVE LEAVES EXTRACTS-BASED FORMULATION IN NEURODEGENERATION: A NETWORK-BASED APPROACH

9^th International Conference on CHEMISTRY AND EURO GREEN CHEMISTRY
May 22-23, 2019 | Rome, Italy

Matteo Micucci, Maria Frosini, Alberto Chiarini and Roberta Budriesi

University of Bologna, Italy

Posters & Accepted Abstracts : J Ind Environ Chem

Abstract:

Despite high clinical heterogeneity, neurodegenerative disorders share many common etiologic features involving increased ROS formation and inflammation. ROS worsen the progression of the disease owing to oxidative damage and impaired mitochondrial function. Moreover, the accumulation of misfolded proteins contributes to the onset and progression of brain inflammatory response, which in turn, increases ROS release and subsequent oxidative stress (OS). Thus, the reduction of ROS levels may be a promising strategy to delay neurodegeneration. In this view many vegetal extracts and natural compounds endowed with antioxidant (but not only) properties are currently under clinical investigations. Pres Phytum® is a nutraceutical product composed by leaves- and flowers-extracts of Olea europaea L. and Hibiscus sabdariffa L., respectively, whose composition has already been characterized by HPLC coupled to a UV-Vis and QqQ-Ms detector. The effects of the components of this natural extracts are widely reported and most of them are mostly due to their antioxidant, antiapoptotic and anti-inflammatory properties. The aim of this study was to assess neuroprotective effects of PRES in in vitro models of OS-mediated injury. Human neuroblastoma SH-SY5Y cells or rat brain slices were treated with hydrogen peroxide and neuronal damage as well as Pres Phytum® neuroprotective effects was assessed. Results showed that Pres Phytum® treatment reverted the increase in sub-diploid, dapi-and annexin V-positive-cells caused by hydrogen-peroxide challenge in SHSY-5Y cells. Furthermore, Pres Phytum® reduced ROS formation, as well as changes in the mitochondrial potential (ΔΨm) and caspase-3, 8 and 9 activity caused by OS. Pres Phytum® neuroprotective effects were confirmed also in rat brain slices. In conclusion, natural compounds still present a great challenge in finding an appropriate treatment to these devastating diseases. In this regards, the present results suggest the possibility of Pres Phytum® as a new preventive strategy for patients with high risk of these pathologies.

Biography:

PDF HTML