+441518081136Hepatoprotective activity of aqueous seed extract of Nigella sativa against highly active antiretroviral therapy induced hepatotoxicity in rats
4th International Congress on Drug Discovery, Designing and Development & International Conference and Exhibition on Biochemistry, Molecular Biology: R&D
November 02-03, 2017 Chicago, USA
Kissi Mudie
Ethiopian Public Health Institute, Ethiopia
Scientific Tracks Abstracts : J Pharmacol Ther Res
Abstract:
Background: Liver is a metabolically active organ responsible for many vital life functions. It performs many activities that are critical for survival. Due to its important activities, the liver is exposed to many insults and is one of the body’s organs most subject to injury. Despite tremendous advances in modern medicine, there are hardly any reliable drugs that protect the liver from damage and/or help in regeneration of hepatic cell. It is, therefore, necessary to search for effective and safe herbal drugs for the treatment of liver disease to replace currently used drugs of doubtful efficacy and safety. Aim: The aim of this study was to investigate the hepatoprotective activity of aqueous extract of Nigella sativa seed in highly active antiretroviral therapy (Lamivudine, Zidovudine and Efavirenz) administered rats. Materials & Methods: Sixty rats weighed between 150200 g were randomly divided into six groups and each group comprised of ten rats. Rats in group I were administered with distilled water. Rats in group II were administered with highly active antiretroviral therapy only. Rats in groups III - VI were administered 100, 200, 400 and 800 mg/kg Nigella sativa plus highly active antiretroviral therapy respectively. The treatments were given orally for 28 consecutive days. On the 29th day, all rats were sacrificed under light diethyl ether anesthesia; blood samples were collected for the assessment of biochemical parameters, while liver tissue was used for histopathological assessment. Results: Serum levels of liver enzymes ALT, AST, ALP, and GGT were significantly (p<0.05) increased and albumin concentration was significantly decreased in animals treated with highly active antiretroviral therapy as compared to the normal control. Histopathological observations also revealed severe damage in the structure of liver tissue in animals administered with highly active antiretroviral therapy. Treatment of highly active antiretroviral therapy exposed animals with Nigella sativa showed marked improvement in both biochemical and histopathological findings. Rise in liver enzymes was almost restored to normal in animals treated with Nigella sativa. Conclusion: Nigella sativa, through its antioxidant activity, effectively protects highly active antiretroviral therapy induced liver toxicity.
Biography:
Kissi Mudie has completed his MSc in Medical Biochemistry from Addis Ababa University, School of Medicine. He is the Director of National Clinical Chemistry. Laboratory, Ethiopian Public Health Institute. He has published more than 14 papers in reputed journals and has been serving as an Associate Researcher
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