Functional and probiotic attributes of Lactococcus lactis subsp. hordinae HB3, a human gut isolated strain
3rd World Congress on Advances in Biotechnology
December 15, 2021 | Webinar
Maryam Idrees
Quaid-I-Azam University, Pakistan
Scientific Tracks Abstracts : J Biochem Biotech
Abstract:
Probiotics have long been recognized to positively modulate several aspects of human health. According to FAO/WHO recommendations, micro-organisms isolated from human gastrointestinal tract could only be used as probiotic’s for humans. Host-adapted autochthonous probiotic strains, have their genomes specialized to facilitate niche-specific fitness. Such strains exhibit higher adaptability and persistence, lower immunostimulatory potential and niche-specific metabolic activity. In the present study, we have discovered human gut-associated, probiotic Lactococcus lactis subsp. hordinae HB3 strain. It was isolation using a LAB specific medium, followed by phenotypical and biochemical characterization. The strain was molecularly identified as Lactococcus lactis subsp. hordinae through 16s rRNA genome sequence analysis. Interestingly, the strain featured important functional and probiotic properties. It negated haemolysis of blood cells and showed susceptibility to most of the tested antibiotics. The strain exhibited tolerance to acid (pH3) and bile salts (0.5%w/v), high enzymatic (lipolytic, amylolytic and proteolytic) potential and significant antimicrobial activity (p<0.05). Therefore, Lactococcus lactis subsp. hordinae HB3 has the potential to be further investigated as an autochthonous putative probiotic strain for human use.
Biography:
Maryam Idrees, PhD scholar from quaid I Azam University Islamabad, Pakistan. and research fellow at national institute of genomics and advanced biotechnology (nigab), national agricultural research centre (narc), Islamabad, Pakistan.
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