+1 (202) 780-3397
Comparative study of GATA3 and CD147 overexpression in urinary bladder carcinoma: Diagnostic and prognostic implications
2nd International Conference on Pathology and Surgical Pathology
July 05-06, 2019 | Paris, France
Nehal Abd El-Ghaffar Heabah, Mohammed Farid Aref, Mohamed Alaa Mokhtar, Mohamed Moustafa Shareef and Ahlam Mohamed Abo El-Enain
Tanta University, Egypt
Posters & Accepted Abstracts : J Clin Path Lab Med
Abstract:
Introduction: Urinary bladder carcinoma is an international
health problem and the most common urologic malignancy,
particularly in males. Many efforts were made to
investigate the role of GATA3 and smoothelin as diagnostic
markers for bladder carcinoma and CD147 (EMMPRIN) as
a prognostic and potentially therapeutic one.
Aim of the work: This study aimed to study the
immunohistochemical expression of GATA3, smoothelin
and CD147 (EMMPRIN) in urinary bladder carcinoma and
its available morphological variants and to correlate the
immunohistochemical results of these markers with the
available clinicopathological parameters.
Materials and methods: Paraffin blocks from 85 cases of
urinary bladder carcinoma patients including: 69 cases
of urothelial carcinoma (UC), 10 cases of squamous cell
carcinoma (SCC), 5 cases of adenocarcinoma and one case of
neuroendocrine small cell carcinoma were stained by GATA3,
smoothelin and CD147 immunohistochemical markers.
Results: GATA3 exhibited high sensitivity (87%) and
specificity (100%) as a diagnostic marker for urothelial
carcinoma. Strong nuclear GATA3 expression (+3) had
been found in all cases of low grade urothelial carcinoma,
plasmacytoid, microcystic, micro-papillary and clear cell
urothelial carcinoma while showed a range of sensitivity as
a marker for urothelial carcinomas with variant morphologic
features as squamous and sarcomatoid differentiation.
A statistically significant relations was found between GATA3
expression and tumor grade and stage, so that reduction in
GATA3 expression was associated with high-grade and muscle
invasive bladder carcinoma. Smoothelin proved an important
diagnostic utility, allowing distinction of the muscularis
propria MP (positive smoothelin expression) from the
muscularis mucosa MM (negative smoothelin expression).
The sensitivity and specificity of smoothelin in detecting
MP invasion in the current study was 100%. Regarding
CD147 expression, positive CD147 staining was significantly
associated with high tumor grade and muscle invasion.
Conclusion: GATA3 acts as a valuable tool for confirming
the urothelial origin of microcystic, micropapillary,
plasmacytoid and clear cell variants of urothelial carcinoma.
Also, decreased GATA3 expression is associated with
high tumor grade and MP invasion. It is important to use
smoothelin immunohistochemistry as a routine in all cases
of TURBT specimens for accurate staging and subsequent
optimal patient management. CD147 positive expression
is significantly related to high grade and advanced stage of
bladder carcinoma.
Biography:
Nehal Abd El-Ghaffar Heabah has completed her Master from Tanta University. She works as an assistant lecturer: Faculty of Medicine, Tanta University, Egypt from 2015-till present. She performed Master thesis entitled: “An immunohistochemical and image analysis study of fragile histidine triad (FHIT) protein expression in colorectal adenoma and carcinoma”, January 2015 and Medical Doctoral thesis entitled: "Immunohistochemical Study of GATA3, Smoothelin and CD147 Expression in Urinary Bladder Carcinoma”, March 2019.
E-mail: nehalghaffar@yahoo.com
PDF HTML