Journal of Cell Science and Mutations

Brain lipid binding protein (BLBP) regulates the proliferation of astrocytes in vitro

Annual Congress on Cell Science, Stem Cell Research & Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA

Haoming Li, Jianbing Qin and Guohua Jin

Medical School of Nantong University, China

Posters & Accepted Abstracts : Adv cel sci tissue cul

Abstract:

The aim of the study is to explore the function of brain lipid binding protein in the proliferation of rats’ astrocytes in vitro. The BLBP overexpressed adenovirus or small interference RNA (siRNA) was used to transfect into astrocytes. 3 days later, the gene and protein expression of BLBP was detected, the proportion of Ki67 and EdU positive cells was measured and the cell cycle was investigated by flow cytometry methods. After treatment of BLBP adenovirus, the expression of BLBP gene was elevated, the exogenous expression of BLBP could be expressed in astrocytes, the proportion of Ki67 and EdU positive cells was also increased, as well as the proportion of cells at S phase. After treatment of BLBP siRNA, the expression of BLBP gene was decreased, the proportion of Ki67 and the cells at S phase was reduced as well. In conclusion, besides as a fatty acids transporter, BLBP also regulates the proliferation of astrocytes in vitro.

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