Biorecognition and biosensing: From big to small

Biorecognition and biosensing: From big to small

Joint Event on 2^nd International Conference on Nanomaterials and Nanotechnology & 22^nd International Conference on Advanced Nanoscience and Nanotechnology
May 16-17, 2019 | Prague, Czech Republic

Yaw-Kuen Li

National Chiao Tung University, Taiwan

Abstract:

Biosensors have drawn much attention because of their great potential to facilitate biomedical research, drug discovery, environmental monitoring and diagnosis of diseases. A powerful bio-detection requires highly specific bio-recognition probes and sensitive elements. Several techniques, such as gold nano-particles (AuNPs), quartz crystal microbalance (QCM) and surface plasmon resonance (SPR), silicon nanowire field-effect transistor (SiNW-FET) and others have been extensively studied to improve the sensitivity of biosensing. Antibody is commonly used to conjugate with a sensing chip for a large molecule detection. Yet, for small molecule detection, new strategies need to be developed. We’ll demonstrate a conventional method, based on protein engineering, to produce a bio-recognition probe and to construct an effective device for quantitatively sensing steroids. We’ll further exhibit an open-sandwich immunorecognition system containing VH and VL of a scFv for small molecules detection. The powerful technique for screening scFv from phage display library will also be discussed.

Biography:

Yaw-Kuen Li received his PhD degree from Tulane University, USA, in 1991. After his postdoctoral research in School of Medicine of Johns Hopkins University, he moved back to Taiwan to start his academic career in 1993. He was promoted to the full professor in 2002. Further, he became the chair of the department (2004-2006) and the dean of college of science of National Chiao Tung University (2014-2017). His primary research interests include three major fields: Enzyme-based catalytic biological reactions, biorecognitionand bio-sensors and solid-state/biological interface chemistry.

E-mail: nctuykl@g2.nctu.edu.tw

PDF HTML