A stem bark extract from Khaya grandifoliola (Meliaceae) C DC. stimulates the chemotactic and phagocytic activity of rat peritoneal macrophages exposed to CCl4

A stem bark extract from Khaya grandifoliola (Meliaceae) C DC. stimulates the chemotactic and phagocytic activity of rat peritoneal macrophages exposed to CCl4

Joint Conference on GLOBAL APPLIED MICROBIOLOGY CONFERENCE & International Congress on MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017 Toronto, Canada

Florence Pare Ngoungoure

University of Yaoundé I, Cameroon

Scientific Tracks Abstracts : Microbiology: Current Research

Abstract:

Khaya grandifoliola is a plant species used in traditional medicine in Cameroon for the treatment of many diseases. This study determines the capacity of chloroform/ methanol (CH2Cl2/CH3OH) (1:1v/v) extract of stem bark of the plant to boost the chemotactic and phagocytic activities of rats macrophages in response to challenge with Saccharomyces cerevisiae. In vitro study showed that Khaya grandifoliola extract (KGE) caused a non-dependent concentration increase in NO production, reduced NBT dye by 75% and enhanced the activity of lysosomal enzyme by 47% at 1 μg/ml. The results of the in vivo study shows that after seven consecutive days of CCL4 exposure, the dose 100 mg/kg of body weight, KGE significantly (p< 0.05) increased the activity of NADH oxidase (1.3 folds), lysosomal enzyme (2 folds); production of H2O2 (2 folds) and NO (7 folds) compared to control group. We conclude that KGE stimulates the phagocytic activity of macrophages both in vitro and in vivo making it a candidate substance for strengthening the immune system.