+441518081136A proteomic approach to identify zeins upon eco-friendly ultrasound-based extraction
15th International Conference on DRUG DISCOVERY AND DEVELOPMENT
December 09, 2021
Laura Darie-Ion
University of Iasi, Romania
Scientific Tracks Abstracts : J Pharmacol Ther Res
Abstract:
In this study we present an enhanced eco-friendly extraction method, including grinding and sieving corn seeds, for prolamins proteins using an ultrasonic extraction methodology. Zein is a type of prolamin storage protein that has a variety of biomedical and industrial applications. Due to the considerable genetic variability and polyploidity of the starting material, as well as the extraction methods used, characterization of the protein composition of zein, requires a combination of different analytical processes. Therefore, we combined modern analytical methods such as mass spectrometry (MS), Sodium dodecyl sulfate polyacrylamide gel electro- phoresis (SDS-PAGE), atomic force microscopy (AFM) or infrared spectroscopy (FTIR-ATR) for a better characterization of the extracted zein. In our studies, the use of ultrasonic homogenizer, 65% ethanol extraction buffer and 710 μm maize granulation yielded the highest protein extraction. MS and SDS-PAGE analysis revealed as main component the α-zein proteins in the maize flour extract. Moreover, fundamental studies based on AFM and FTIR-ATR approaches were ap-plied to investigate the effect of different ethanol solutions on the self- assembled structures of zein protein. This research leads to a better understanding of zeins content, critical for develop-ing new applications of zein in food and pharmaceutical industries, such as biocompatible med-ical vehicles based on polyplexes complex nanoparticles of zein with antimicrobial or drug de-livery properties.
Biography:
Laura Darie Ion has completed her PhD in 2015 at Chemistry Department, Alexandru Ioan Cuza University of Iasi, Romania and currently, she is postdoctoral researcher at the same University. During the PhD and postdoctoral studies, she has completed several research internships at University of Konstanz, Germany and published more than 13 papers in reputed international journals.
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