Mini Review - Journal of Clinical Immunology Research (2023) Volume 6, Issue 2

Genotyping techniques: Tools for analyzing genetic variations.

Department of Entomology, University of Massachusetts, Amherst, USA

*Corresponding Author:

Jeong Zhang
Department of Entomology
University of Massachusetts, Amherst, USA
E-mail: zhang@ent.umass.edu

Received: 04-Apr-2023, Manuscript No. AACIR-23-98595; Editor assigned: 07-Apr-2023, Pre QC No. AACIR-23-98595(PQ); Reviewed: 21-Apr-2023, QC No. AACIR-23-98595; Revised: 24-Apr-2023, Manuscript No. AACIR-23-98595(R); Published: 28-Apr-2023, DOI: 10.35841/aacir-6.2.145

Citation: Zhang J. Genotyping techniques: Tools for analyzing genetic variations. J Clin Immunol. 2023;6(2):145

Abstract

Introduction

Genotyping techniques play a crucial role in the field of genetics and molecular biology, enabling the identification and analysis of genetic variations within individuals or populations. These techniques provide valuable information about an organism's genotype, which refers to its specific genetic makeup. By understanding an organism's genotype, researchers can study the relationship between genetic variations and various phenotypic traits or diseases. In this article, we will explore some commonly used genotyping techniques and their applications in research and clinical settings [1].

PCR is a widely used genotyping technique that amplifies specific DNA sequences of interest. It allows researchers to obtain multiple copies of a target DNA region, making it easier to analyze and detect genetic variations. PCR-based genotyping can be performed using different variations of the technique, such as allele-specific PCR, Restriction Fragment Length Polymorphism (RFLP), and quantitative PCR (qPCR). PCR-based genotyping is commonly used for Single Nucleotide Polymorphism (SNP) analysis, which involves identifying differences in a single DNA base pair [2].

Restriction fragment length polymorphism (RFLP)

RFLP is a genotyping technique that takes advantage of naturally occurring genetic variations that result in different DNA fragment sizes. It involves digesting DNA samples with specific restriction enzymes that recognize and cut DNA at specific DNA sequences. The resulting DNA fragments are then separated using gel electrophoresis and visualized to identify differences in fragment sizes among individuals or populations. RFLP is particularly useful for detecting genetic variations associated with diseases or population studies SNPs are the most common type of genetic variation, where a single nucleotide (A, T, C, or G) differs between individuals. SNP genotyping techniques enable the identification and analysis of these variations. Methods such as SNP arrays and TaqMan assays use specific probes or primers that hybridize to target DNA regions containing the SNP of interest. The presence or absence of specific SNP alleles can be determined through various detection methods, such as fluorescence-based techniques or mass spectrometry.

Microsatellites, also known as Short Tandem Repeats (STRs), are repetitive DNA sequences that vary in the number of repeats between individuals. Microsatellite analysis involves PCR amplification of these short repeat regions, followed by fragment analysis using capillary electrophoresis. By comparing the sizes of the amplified fragments, researchers can identify variations in the number of repeats and determine an individual's genotype. Microsatellite analysis is widely used in forensic genetics, population genetics, and parentage testing [3].

DNA sequencing provides a comprehensive analysis of an organism's genetic information by determining the precise order of nucleotides in a DNA molecule. While traditional Sanger sequencing is widely used for targeted sequencing, Next-Generation Sequencing (NGS) technologies have revolutionized genotyping by enabling high-throughput sequencing of entire genomes or specific genomic regions. NGS-based genotyping allows for the detection of various types of genetic variations, including SNPs, insertions, deletions, and structural variants.

Allele-Specific Oligonucleotide Hybridization (ASO) is a genotyping technique that uses short DNA probes that are complementary to specific target sequences. These probes are designed to be specific to different alleles or variations of a target gene or SNP. By hybridizing these probes with the DNA sample, researchers can identify the presence or absence of specific alleles based on the probe's binding pattern. ASO genotyping is commonly used in clinical settings for the detection of disease-associated mutations or pharmacogenetic studies [4].

An environment favourable to immunogenicity is created by anthracyclines. According to the researchers, this drug encourages antigen uptake and presentation by dendritic cells when killing cancer cells, triggering a T-cell response that can decrease tumours. Hence, the efficacy of immunotherapy depends on the activation of tumour-killing T-cells [5].

Conclusion

Genotyping techniques have revolutionized our understanding of the human genome and have provided valuable insights into the genetic basis of diseases and other traits. Microarray analysis, DNA sequencing, and PCR-based methods are all commonly used genotyping techniques that can be used to detect a wide range of genetic variations. These techniques have many applications in research and clinical settings, including disease diagnosis, drug development, and personalized medicine.

References

1. Wolska K, Szweda P. Genotyping techniques for determining the diversity of microorganisms. Genetic diversity in microorganisms. In Tech Croatia. 2012:53-94.

Indexed at, Google Scholar, Cross Ref

2. Olschewsky A, Hinrichs D. An overview of the use of genotyping techniques for assessing genetic diversity in local farm animal breeds. Animals. 2021;11(7):2016.

Indexed at, Google Scholar, Cross Ref

3. Shen W, Tian Y, Ran T, et al. Genotyping and quantification techniques for single-nucleotide polymorphisms. TrAC Trends in Anal Chem. 2015;69:1-3.

Indexed at, Google Scholar, Cross Ref

4. Clark JM, Lee SH, Kim HJ, et al. DNA‐based genotyping techniques for the detection of point mutations associated with insecticide resistance in Colorado potato beetle Leptinotarsa decemlineata. Pest Manag Sci. 2001;57(10):968-74.

Indexed at, Google Scholar, Cross Ref

5. Ye S, Dhillon S, Ke X, et al. An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 2001;29(17):e88.

Indexed at, Google Scholar, Cross Ref